自噬相关的环状RNA circ0020123在卵巢癌中的诊断价值  

Diagnostic value of autophagy-related circular RNA circ_0020123 in ovarian cancer

作　　者：王亚男 褚雪莲 张风卫[2] 顾春川 Wang Yanan;Chu Xuelian;Zhang Fengwei;Gu Chunchuan(Hangzhou Linping District Maternal and Child Health Hospital/Linping Branch of the Obstetrics and Gynecology Hospital Affiliated to Zhejiang University School of Medicine,Hangzhou 311199,China;Hangzhou Red Cross Hospital,Hangzhou 310003,China;Shulan Hospital of Xiacheng District,Hangzhou 310022,China)

机构地区：[1]浙江省杭州市临平区妇幼保健院,杭州311199 [2]杭州市红十字会医院,杭州310003 [3]浙江省杭州市下城区树兰(杭州)医院,杭州310022

出　　处：《中华内分泌外科杂志》2022年第5期622-626,共5页Chinese Journal of Endocrine Surgery

基　　金：杭州市医药卫生科技项目(OO20190070)。

摘　　要：目的探讨卵巢癌(ovarian cancer,OC)中环状RNA circ_0020123表达水平,进而评估circ_0020123水平对卵巢癌的诊断价值。方法收集2016年10月至2017年3月在杭州市临平区妇幼保健院进行诊治的卵巢癌患者55例,收集血清进行高通量基因表达测序分析。采用实时荧光定量PCR技术测定卵巢癌患者和健康对照组血清中circ_0020123水平,通过绘制受试者工作特征曲线(receiver operating characteristic curve,ROC),评估circ_0020123水平对卵巢癌的诊断效能。通过t检验探讨circ_0020123表达与卵巢癌患者临床特征的相关性。绘制生存曲线分析卵巢癌患者血清circ_0020123水平与预后生存的关系。Western blot检测自噬相关蛋白LC3-Ⅱ/LC3-Ⅰ、p62表达水平。结果与健康人血清(1.00±0.25)相比,circ_0020123在卵巢癌患者血清(1.24±0.23)中表达明显上调(t=5.23,P<0.001)。ROC曲线显示circ_0020123对卵巢癌标本的检测具有较高的诊断性能(AUC=0.7579,P<0.001)。t检验与logistics综合分析显示circ_0020123高表达明显与FIGO分期(t=5.46,P<0.001)和肿瘤大小(t=6.37,P<0.001)相关。生存资料测定显示,circ_0020123高表达组的5年总生存期明显短于circ_0020123低表达组(P=0.019)。在细胞中敲减circ_0020123能下调自噬相关蛋白LC3-Ⅱ/LC3-Ⅰ的表达(0.45±0.05)(t=9.31。P=0.001),且上调p62的表达(1.94±0.11)(t=12.62,P<0.001)。结论 circ020123是一个重要的卵巢癌相关环状RNA, 为卵巢癌患者提供了一个潜在的诊断、预后的生物标志物和治疗靶点。Objective To investigate the expression level of circular RNA circ_0020123 in ovarian cancer(OC),and then to evaluate the diagnostic value of circ_0020123 level in ovarian cancer.Methods Fifty-five ovarian cancer patients who were diagnosed and treated in Hangzhou Linping District Maternal and Child Health Hospital from Oct.2016 to Mar.2017 were selected,and serum was collected for high-throughput gene expression sequencing analysis.The circ_0020123 level in serum of ovarian cancer patients and healthy controls was determined by real-time quantitative PCR,and the diagnostic efficacy of circ_0020123 level on ovarian cancer was evaluated by drawing receiver operating characteristic curve(ROC).The correlation between the expression of circ_0020123 and the clinical characteristics of ovarian cancer patients was investigated by t-test.The survival curve was drawn to analyze the relationship between the serum circ_0020123 level and the prognosis and survival of ovarian cancer patients.Western blot was used to detect the expression levels of autophagy-related proteins LC3-II/LC3-I and p62.Results Compared with the serum of healthy people(1±0.25),the expression of circ_0020123 was significantly up-regulated in the serum of ovarian cancer patients(1.24±0.23)(t=5.23,P<0.001).The ROC curve showed that circ_0020123 had high diagnostic performance for the detection of ovarian cancer specimens(AUC=0.7579,P<0.001).Comprehensive analysis of t test and logistic analysis showed that the high expression of circ_0020123 was significantly correlated with FIGO stage(t=5.46,P<0.001)and tumor size(t=6.37,P<0.001).The measurement of survival data showed that the 5-year overall survival of the circ_0020123 high expression group was significantly shorter than that of the circ_0020123 low expression group(P=0.019).Knockdown of circ_0020123 in cells down-regulated the expression of autophagy-related proteins LC3-II/LC3-I(0.45±0.05)(t=9.31,P=0.001),and up-regulated the expression of p62(1.94±0.11)(t=12.62,P<0.001).Conclusion The results o

关 键 词：circ0020123 卵巢癌 FIGO分期 自噬 

分 类 号：R737.31[医药卫生—肿瘤]