GSTP1和PLCE1基因多态性与原发性食管癌的相关性研究  

Association of GSTP1 and PLCE1 gene polymorphisms with primary esophageal cancer

作　　者：韩文杰[1] 李伟艳 何章彪[3] Han Wenjie;Li Weiyan;He Zhangbiao(Department of Oncology,Shangqiu First People′s Hospital,Shangqiu,Henan 476100,China;Department of Laboratory Medicine,Shangqiu Municipal Hospital,Shangqiu,Henan 476000,China;Shangqiu Medical College,Shangqiu,Henan 476299,China)

机构地区：[1]商丘市第一人民医院肿瘤科,河南476100 [2]商丘市立医院检验科,河南476000 [3]商丘医学高等专科学校,河南476299

出　　处：《中华医学遗传学杂志》2022年第11期1283-1289,共7页Chinese Journal of Medical Genetics

基　　金：河南省高等学校重点科研项目(19A320028)。

摘　　要：目的研究谷胱甘肽S转移酶P1(GSTP1)和磷脂酶Cε1(PLCE1)基因多态性对原发性食管癌易感性的影响及其与环境因素的交互作用。方法选取原发性食管癌患者和健康人群各162例为样本,采集性别、年龄、烟酒史和食管癌家族史等信息,并检测其GSTP1基因A105G位点和PLCE1基因rs3765524、rs2274223、rs3781264位点的单核苷酸多态性,建立Logistic回归模型,分析食管癌风险因素及各因素的交互作用。结果食管癌组吸烟史、食管癌家族史和热烫饮食人群占比高于对照组,差异有统计学意义(P<0.05);条件Logistic回归分析显示吸烟、食管癌家族史和PLCE1 rs2274223 GG基因型为食管癌的风险因素(P<0.05),GSTP1 A105G AG/GG基因型是食管癌的保护因素(P<0.05);两因素交互模型中GSTP1 A105G AA和PLCE1 rs2274223 GG基因型均与吸烟具有交互作用,食管癌患病风险分别升高83.6%和85.7%(P<0.05);GSTP1 A105G AA基因型、PLCE1 rs2274223 GG基因型和吸烟为最佳三因素交互模型,食管癌患病风险可升高244.0%(P<0.05);四因素交互模型分析显示同时存在GSTP1 A105G AA基因型、PLCE1 rs2274223 GG基因型、吸烟和食管癌家族史的人群食管癌患病风险可升高264.4%(P<0.05)。结论GSTP1基因A105G位点AG和GG基因型为食管癌保护因素,PLCE1基因rs2274223位点GG基因型为食管癌风险因素,且均可与吸烟产生交互作用,对食管癌易感性造成影响。Objective To assess the association of polymorphisms of glutathione S-transferase P1(GSTP1)and phospholipase C epsilon-1(PLCE1)genes with the susceptibility of primary esophageal cancer and their interaction with environmental factors.Methods 162 patients with primary esophageal cancer and 162 healthy controls were recruited in this cross-sectional study.Basic information such as gender,age,history of smoking and alcohol consumption and family history of esophageal cancer were collected.Single nucleotide polymorphisms at A105G locus of GSTP1 gene and at rs3765524,rs2274223 and rs3781264 loci of PLCE1 gene were detected.A logistic regression model was established to analyze the risk factors of esophageal cancer and the interaction among the factors.Results The proportions of individuals with smoking history,family history of esophageal cancer and hot diet in esophageal cancer group were higher than those in the control group(P<0.05).Conditional Logistic regression analysis showed that smoking,family history of esophageal cancer and GG genotype at the rs2274223 locus of PLCE1 gene were the risk factors for esophageal cancer(P<0.05),and AG/GG genotypes at the A105G locus of GSTP1 gene were the protective factors for esophageal cancer(P<0.05).In the two-factor interaction model,both AA genotype at A105G locus of GSTP1 gene and GG genotype at rs2274223 locus of PLCE1 gene had an interaction with smoking,and the risk of esophageal cancer has increased by 83.6%and 85.7%,respectively(P<0.05).AA genotype at A105G locus of GSTP1 gene,GG genotype at rs2274223 locus of PLCE1 gene and smoking constituted the best three-factor interaction model,and the risk of esophageal cancer has increased by 244.0%(P<0.05).Four-factor interaction model analysis showed that the risk of esophageal cancer among individuals with AA genotype at A105G locus of GSTP1 gene,GG genotype at rs2274223 locus of PLCE1 gene,smoking and family history of esophageal cancer has increased by 264.4%(P<0.05).Conclusion The AG and GG genotypes at the A105G locus

关 键 词：食管癌 谷胱甘肽S转移酶P1 磷脂酶Cε1 单核苷酸多态性 交互作用 

分 类 号：R735.1[医药卫生—肿瘤]