上海市崇明区中华按蚊杀虫剂抗性及kdr、ace-1等位基因突变研究  

Insecticide resistance and kdr,ace-1 allele mutations of Anopheles sinensis in Chongming District,Shanghai

作　　者：魏子昕 曾文博 杨丽敏 杭添 方圆 施文琦 张仪 WEI Zixin;ZENG Wenbo;YANG Limin;HANG Tian;FANG Yuan;SHI Wenqi;ZHANG Yi(National Institute of Parasitic Diseases,Chinese Center for Disease Control and Prevention(Chinese Center for Tropical Diseases Research),NHC Key Laboratory of Parasite and Vector Biology,WHO Collaborating Center for Tropical Diseases,National Center for International Research on Tropical Diseases,Shanghai 200025,China;School of Global Health,Chinese Center for Tropical Diseases Research,Shanghai Jiao Tong University School of Medicine,Shanghai 200025,China)

机构地区：[1]中国疾病预防控制中心寄生虫病预防控制所(国家热带病研究中心),国家卫生健康委员会寄生虫病原与媒介生物学重点实验室,WHO热带病合作中心,国家级热带病国际联合研究中心,上海200025 [2]上海交通大学医学院国家热带病研究中心全球健康学院,上海200025

出　　处：《上海预防医学》2022年第10期988-994,共7页Shanghai Journal of Preventive Medicine

基　　金：国家科技基础资源调查专项(2017FY101203);国家自然科学基金(32161143036);上海市第五轮公共卫生体系建设三年行动计划重点学科项目(GWV-10.1-XK13)。

摘　　要：【目的】研究上海市崇明区传疟媒介中华按蚊对有机磷类杀虫剂和拟除虫菊酯类杀虫剂的抗性情况及部分靶标抗性基因突变情况。【方法】在上海市崇明区不同采样点采集3个中华按蚊自然种群,用世界卫生组织(WHO)推荐的接触筒法测试蚊虫杀虫剂抗性,使用聚合酶链式反应(PCR)技术检测成蚊的ace‑1、击倒抗性(kdr)等位基因频率,采用直线相关回归方法探索杀虫剂抗性与kdr等位基因频率的相关性。【结果】近自然保护区种群对溴氰菊酯敏感,24 h死亡率为100.00%;养殖场密集区种群对溴氰菊酯和高效氯氟氰菊酯呈抗性水平,24 h死亡率分别为48.00%、57.50%;城乡接合区种群对溴氰菊酯和高效氯氟氰菊酯产生可疑抗性24 h死亡率为84.40%和86.00%;对杀螟硫磷呈现抗性水平,24 h死亡率为55.67%。kdr基因1014位点存在3种基因突变,检测到7种突变基因型,以L1014/L1014F、L1014F/L1014F为主,养殖密集区种群kdr等位基因突变频率最高(52.68%)。ace‑1基因119位点存在1种突变,检测到3种基因型,以G119S突变为主。中华按蚊kdr抗性等位基因频率与其对高效氯氰菊酯的死亡率呈负相关(r2=0.930,P=0.036);4个不同生境中华按蚊种群ace‑1抗性等位基因频率与其对杀螟硫磷的死亡率呈负相关(r2=0.996,P=0.038)。【结论】杀虫剂抗性等位基因突变频率与蚊虫杀虫剂抗性水平产生有关,因此应继续全面监测崇明区中华按蚊抗性水平,为当地蚊虫杀虫剂抗性治理提供依据。[Objective]To investigate the resistance to pyrethroids,beta-cyhalothrin and fenitrothion as well as the frequencies of kdr and ace‑1 alleles in Anopheles sinensis from three geographical populations in Chongming District,Shanghai.[Methods]The contact tube method recommended by WHO was used to determine mosquito resistance.The frequencies of kdr and ace‑1 alleles in adult mosquitoes were determined by PCR amplification.The correlation between the frequencies of kdr alleles and insecticide resistance was analyzed by linear regression method.[Results]The mosquito population near natural reserve(NF)was sensitive to deltamethrin,and the mortality rate was 100.00%at 24 h.The mosquito population with intensive breeding farms(HX)showed resistance to deltamethrin and beta-cyhalothrin,and the 24 h mortality was 48.00%and 57.50%,respectively.The 24 h mortality rate of the mosquito population with urban-rural integration area(AS)with suspected resistance to deltamethrin and beta-cyhalothrin was 84.40%and 86.00%.The 24 h mortality rate to Fenitrothion was 55.67%.There were 3 mutations in kdr gene locus 1014,and 7 mutated genotypes were detected,mainly L1014/L1014F and L1014F/L1014F.The mutation frequency of kdr allele in HX population was the highest(52.68%).There was one mutation at site 119 of ace‑1 gene,and three genotypes were detected,most of which were G119S mutation.There was a significant negative correlation between the frequency of kdr resistance allele and the mortality rate of beta-cypermethrin in An.sinensis populations(r2=0.930,P=0.036).There was a significant negative correlation between the frequency of ace‑1 resistance allele and the mortality rate of fenitrothion in An.sinensis populations from four habitats(r2=0.996,P=0.038).[Conclusion]The mutation frequency of insecticide resistance allele is associated with the development of mosquito resistance level.Therefore,comprehensive monitoring of resistance levels of An.sinensis in Chongming area should be continued to provide a basis for local mosquito

关 键 词：中华按蚊 抗药性 拟除虫菊酯类杀虫剂 有机磷类杀虫剂 击倒抗性 等位基因频率 

分 类 号：R38[医药卫生—医学寄生虫学]