红花NAC基因家族鉴定及干旱胁迫响应分析  

Identification of Carthamus tinctorius NAC gene family and analysis of drought stress response

作　　者：詹鹏 钟祖昌 向妮艳 覃瑞[1] 江雄波 刘虹[1] ZHAN Peng;ZHONG Zu-chang;XIANG Ni-yan;QIN Rui;JIANG Xiong-bo;LIU Hong(Hubei Provincial Key Laboratory for Protection and Application of Special Plant Germplasm in Wuling Area of China,Key Laboratory of State Ethnic Affairs Commission for Biological Technology,College of Life Sciences,South-Central Minzu University,Wuhan 430074,China;Hubei Ecology Polytechnic College,Wuhan 430200,China)

机构地区：[1]中南民族大学生命科学学院,生物技术国家民委重点实验室,武陵山区特色资源植物种质保护与利用湖北省重点实验室,湖北武汉430074 [2]湖北生态职业技术学院,湖北武汉430200

出　　处：《中国中药杂志》2022年第20期5520-5529,共10页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(32072123)。

摘　　要：NAC(NAM/ATAF/CUC)转录因子是植物最大的转录基因家族之一,在植物应对干旱胁迫中具有重要作用。为了研究NAC基因家族在红花中的数量及功能,该文以红花的全基因组数据为基础,利用生物信息学手段对红花NAC基因家族成员进行鉴定,并分析了其理化性质、染色体定位、系统进化、基因结构、保守结构域及保守基序。同时,利用qRT-PCR方法分析了4个NAC基因在干旱胁迫处理不同时间后的转录水平。结果表明,红花NAC基因家族有87个成员,不均匀分布在11条染色体上,其中第12号染色体不存在NAC基因;87个NAC基因氨基酸数目为103~974个,CDS数目为3~9个;进化分析可将其分为17个亚家族;保守结构域分析大多数基因含有5个保守的亚结构域A~E;保守基序分析发现,motif2在NAC基因中最保守。表达模式分析发现干旱胁迫不同处理时间后,4个与抗旱相关的NAC基因在叶片中的转录水平均呈上调趋势,推测这4个NAC基因可能与红花抗旱性有关。该研究为红花NAC转录因子进一步功能研究奠定了理论基础,为培育耐旱性红花品种提供参考依据。The NAC(NAM/ATAF/CUC) transcription factors are members of the largest transcriptional gene family in plants and play an essential role in the response of plants to drought stress. To identify the number and function of the NAC gene family in Carthamus tinctorius, the present study adopted bioinformatics methods to identify NAC gene family members based on the whole genome data of C. tinctorius, and analyzed their physicochemical properties, chromosomal location, phylogenetic relationship, gene structure, conserved domain, and conserved motif. Meanwhile, the real-time fluorescence-based quantitative RT-PCR(qRT-PCR) was used to analyze the transcription level of four NAC genes under drought stress in different time. The results showed that C. tinctorius contained 87 NAC genes unevenly distributed on 11 chromosomes, while no NAC gene was found on chromosome 12. The encoded proteins were 103-974 amino acids and the number of CDS ranged from 3 to 9. According to the phylogenetic relationships, 87 NAC genes were clustered into17 subfamilies. The analysis of conserved domains and motifs revealed that most of the genes contained five conserved subdomains, A-E and motif2 was the most conserved among NAC genes. The expression pattern analysis showed that the transcription levels of four NAC genes related to drought resistance were all up-regulated after drought stress treatment for different time, suggesting that these four NAC genes may be related to drought resistance of C. tinctorius. This study is expected to provide a theoretical basis for further functional analysis of NAC transcription factors in C. tinctorius and references for the cultivation of drought-tolerant C. tinctorius varieties.

关 键 词：红花 NAC基因家族 干旱胁迫 转录水平 

分 类 号：S567.219[农业科学—中草药栽培]