Inhibiting phosphatase and actin regulator 1 expression is neuroprotective in the context of traumatic brain injury  

作　　者：Yao Jing Lin Zhang Shi-Wen Chen Yan Guo Shi-Ming Ju Fang Yuan Hao Chen Dian-Xu Yang Heng-Li Tian Zhi-Ming Xu Jun Ding 

机构地区：[1]Department of Neurosurgery,Shanghai Sixth People’s Hospital Affiliated to Shanghai Jiao Tong University School of Medicine,Shanghai,China

出　　处：《Neural Regeneration Research》2023年第7期1578-1583,共6页中国神经再生研究（英文版）

基　　金：supported by the National Natural Science Foundation of China,Nos.81501048(to JD),81801236(to ZMX),81974189(to HLT);Shanghai 6th People’s Hospital Research Fund,No.ynlc201808(to JD).

摘　　要：Studies have found that the phosphatase actin regulatory factor 1 expression can be related to stroke,but it remains unclear whether changes in phosphatase actin regulatory factor 1 expression also play a role in traumatic brain injury.In this study we found that,in a mouse model of traumatic brain injury induced by controlled cortical impact,phosphatase actin regulatory factor 1 expression is increased in endothelial cells,neurons,astrocytes,and microglia.When we overexpressed phosphatase actin regulatory factor 1 by injection an adeno-associated virus vector into the contused area in the traumatic brain injury mice,the water content of the brain tissue increased.However,when phosphatase actin regulatory factor 1 was knocked down,the water content decreased.We also found that inhibiting phosphatase actin regulatory factor 1 expression regulated the nuclear factor kappa B signaling pathway,decreased blood-brain barrier permeability,reduced aquaporin 4 and intercellular adhesion molecule 1 expression,inhibited neuroinflammation,and neuronal apoptosis,thereby improving neurological function.The findings from this study indicate that phosphatase actin regulatory factor 1 may be a potential therapeutic target for traumatic brain injury.

关 键 词：apoptosis aquaporin 4 blood brain barrier intercellular adhesion molecule 1 NEUROINFLAMMATION nuclear factor kappa B OCCLUDIN phosphatase and actin regulator-1 traumatic brain injury zonula occludens 1 

分 类 号：R651.15[医药卫生—外科学]