深圳红树林沉积物可培养细菌多样性及其生物活性筛选  

Diversity and Bioactivity Screening of Culturable Bacteria in Shenzhen Mangrove Sediments

作　　者：龙梦 樊慧敏 夏洪丽 汪志文 喻大鹏 夏立群 鲁义善[1,2] LONG Meng;FAN Hui-min;XIA Hong-li;WANG Zhi-wen;YU Da-peng;XIA Li-qun;LU Yi-shan(Shenzhen Institute of Guangdong Ocean University/Guangdong Provincial Engineering Research Center for Aquatic Animal Health Assessment/Shenzhen Public Service Platform for Evaluation of Marine Economic Animal Seedings,Shenzhen 518120,China;Fisheries College of Guangdong Ocean University/Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture,Zhanjiang 524088,China)

机构地区：[1]广东海洋大学深圳研究院/广东省水生动物健康评估工程技术研究中心/深圳市海水经济动物种苗健康评价公共技术服务平台,广东深圳518120 [2]广东海洋大学水产学院/广东省水产动物病害防控与健康养殖重点实验室,广东湛江524088

出　　处：《广东海洋大学学报》2022年第6期88-96,共9页Journal of Guangdong Ocean University

基　　金：深圳市科技计划项目(20180507183240459);深圳市大鹏新区科技创新和产业发展专项资金资助项目(PT202101-24)。

摘　　要：【目的】研究广东深圳红树林沉积物可培养细菌多样性,筛选潜在生物活性菌株及水产病原菌拮抗菌。【方法】采用稀释涂布法分离纯化深圳红树林沉积物中可培养细菌,基于16S rRNA序列分析细菌物种多样性,通过PCR扩增聚酮合酶基因、非核糖体肽合成酶基因检测菌株产次级代谢产物潜力,并采用点种法筛选水产病原菌拮抗菌。【结果】获得177株细菌,隶属于4门20属27种,芽孢杆菌属(Bacillus)是优势菌属,6株细菌为潜在新物种,55株细菌的基因组DNA扩增出至少1种生物合成基因,其中有7株细菌对水产病原菌有拮抗活性。【结论】深圳红树林沉积物可培养细菌资源丰富,有应用于活性次级代谢产物发掘和水产病害绿色防控的巨大潜力。【Objective】To study the diversity of culturable bacteria in mangrove sediments in Shenzhen,Guangdong,and screen potential bioactive strains and antagonistic bacteria of aquatic pathogens.【Methods】The bacteria of mangrove sediments in Shenzhen were isolated and purified by dilution-coating method,and the diversity of bacteria was analyzed based on 16S rRNA sequences,and the polyketide synthase gene and the non-ribosomal peptide synthase gene were amplified by PCR to detect bacteria's potential to produce secondary metabolites,in addition,the antagonistic bacteria of aquatic pathogens were screened by dot-inoculating method.【Results】177 bacteria were obtained,belonging to 4 phyla,20 genera and 27 species.Bacillus was the dominant genus,6 bacteria were potential new species,and at least 1 biosynthetic gene was amplified from the genomic DNA of 55 bacteria.Seven bacteria had antagonistic activity against aquatic pathogens.【Conclusion】Shenzhen mangrove sediments are rich in culturable bacteria and have great potential in the discovery of active secondary metabolites and the application in green prevention and control of aquatic diseases.

关 键 词：红树林沉积物 细菌多样性 拮抗活性 水产病原菌 生物合成基因 

分 类 号：X172[环境科学与工程—环境科学] Q938.15[生物学—微生物学]