基于高通量测序分析黔中金荞麦内生菌及其根际土壤微生物多样性  

Diversity Analysis of Fagopyrum dibotrys Qian Zhong Endophytes and Rhizosphere Soil Microorganism by High-Throughput Sequencing

作　　者：张涛[1] 周思旋 唐远江 陶小艳 卢昱希 赵宇 史开志 李婷 杨粤黔 Zhang Tao;Zhou Sixuan;Tang Yuanjiang;Tao Xiaoyan;Lu Yuxi;Zhao Yu;Shi Kaizhi;Li Ting;Yang Yueqian(Animal Husbandry and Veterinary Research Institute,Guizhou Academy of Agricultural Sciences,Guiyang 550005,China)

机构地区：[1]贵州省农业科学院畜牧兽医研究所,贵州贵阳550005

出　　处：《山东农业科学》2022年第11期70-75,共6页Shandong Agricultural Sciences

基　　金：贵州省农业攻关计划项目“金荞麦活性物质提取工艺的优化与猪用功能性饲料添加剂的研发”(黔科合支撑[2020]1Y082号);贵州省科技计划项目“山地生态养猪产业化技术创新及示范推广服务企业行动计划”(黔科合服企[2018]4007号)。

摘　　要：为探究贵州药饲兼用牧草黔中金荞麦内生菌及其根际土壤微生物的群落组成与结构,本试验采用高通量测序技术对其测序并进行生物信息学分析。结果表明,内生细菌有效序列524889条,聚类注释得到OTUs 1675条;内生真菌有效序列569064条,注释得到OTUs 2076条。黔中金荞麦茎叶中内生细菌Chao 1指数、ACE指数、Simpson指数、Shannon指数均低于根部,而真菌的4个指数均高于根部。根际土壤微生物各指数均高于黔中金荞麦茎叶和根部,微生物种类最为复杂、数量最多。黔中金荞麦茎叶和根内生细菌群落以蓝藻菌门(Cyanobacteria)为优势菌门,相对丰度分别为75.23%和63.83%,根际土壤为变形菌门(Proteobacteria),相对丰度为36.12%;真菌门水平上,在茎叶、根及根际土中以子囊菌门(Ascomycota)和担子菌门(Basidiomycota)为优势菌门。在属水平上,茎叶与根内生细菌中变形菌门未知属(unidentified_Chloroplast)相对丰度最高,分别占所有菌属的75.23%和63.82%,根际土壤中为赫黄嗜盐囊菌属(Haliangium),占所有菌属的2.96%;茎叶内生真菌枝孢菌属(Cladosporium)相对丰度最高(16.10%),根中灰球菌属(Bovista)相对丰度最高(17.67%),根际土壤中镰孢菌属(Fusarium)相对丰度最高(21.09%)。综上,黔中金荞麦内生菌及土壤微生物群落多样性存在差异,为进一步对黔中金荞麦的资源保护和内生菌的开发利用提供了理论依据。In order to explore the community composition and structure of endophytes and the rhizosphere soil microbial communities of Fagopyrum dibotrys Qian Zhong,a high quality forage grass used for both medicine and forage in Guizhou Province,the high-throughput sequencing method was used for sequencing and bioinformatics analysis.The results showed that 524889 effective sequences and 1675 operational taxonomic units(OTUs)were obtained for endophytic bacteria,while 569064 effective sequences and 2076 OTUs were obtained for endophytic fungi.The Chao1,ACE,Simpson and Shannon indexes of bacteria in stems and leaves of Fagopyrum dibotrys Qian Zhong were lower than those in roots,while those of fungi were higher compared with roots.The above indexes of rhizosphere soil microorganism were higher than those of stems,leaves and roots of Fagopyrum dibotrys Qian Zhong,which indicated that the microbial species were the most complex and abundant.Cyanobacteria was the dominant phylum of endophytic bacteria communities in stems and leaves and roots of Fagopyrum dibotrys Qian Zhong with relative abundance as 75.23%and 63.83%respectively,while Proteobacteria was the dominant one in rhizosphere soil with relative abundance as 36.12%;at the phylum level of fungi,Ascomycota and Basidiomycota were dominant in stems,leaves,roots and rhizosphere soil.At the genus level of endophytic bacteria,the unidentified_Chloroplast of Proteobacteria had the highest relative abundance,and accounted for 75.23%in stems and leaves and 63.82%in roots of all bacterial genera,respectively,while Haliangium was the dominant one in rhizosphere soil accounting for 2.96%.The dominant fungi genus was Cladosporium in stems and leaves,Bovista in roots and Fusarium in soil with the highest relative abundance as 16.10%,17.67%and 21.09%,respectively.In conclusion,the diversity of endophytes and soil microbial communities of Fagopyrum dibotrys Qian Zhong were different,which could provide theoretical bases for further conservation,development and utilization of Fagopyrum

关 键 词：黔中金荞麦 高通量测序 内生菌 16S rRNA ITS 微生物多样性 

分 类 号：S476.1[农业科学—农业昆虫与害虫防治]