Comprehensive analysis of the relationship between cuproptosisrelated genes and esophageal cancer prognosis  

作　　者：Hao Xu Qian-Cheng Du Xin-Yu Wang Ling Zhou Jian Wang Ying-Ying Ma Meng-Yao Liu Hua Yu 

机构地区：[1]General Surgery,Shanghai Xuhui Central Hospital,Shanghai 200031,China [2]Thoracic Surgery,Shanghai Xuhui Central Hospital,Shanghai 200031,China [3]General Surgery,Shanghai Fourth People’s Hospital,School of Medicine,Tongji University,Shanghai 200434,China

出　　处：《World Journal of Clinical Cases》2022年第33期12089-12103,共15页世界临床病例杂志

基　　金：Supported by the Shanghai Fourth People’s Hospital Affiliated to Tongji University School of Medicine Talent Boosting Plan,No.SY-XKZT-2020-3007.

摘　　要：BACKGROUND Esophageal cancer is one of the most common malignant tumors of the digestive system,with a 5-year survival rate of 15%to 50%.Cuproptosis,a unique kind of cell death driven by protein lipoylation,is strongly connected to mitochondrial metabolism.The clinical implications of cuproptosis-related genes in esophageal cancer,however,are mainly unknown.AIM To identify cuprotosis-related genes that are differentially expressed in esophageal cancer and investigate their prognostic significance.METHODS With|log fold change|>1 and false discovery rate<0.05 as criteria,the Wilcox test was used to evaluate the differentially expressed genes between 151 tumor tissues and 151 normal esophageal tissues.Cuproptosis-related genes were selected to be linked with prognosis using univariate Cox regression analysis.Genes were separated into high-and low-expression groups based on their cutoff value of gene expression,and the correlation between the two groups and overall survival or progression-free survival was investigated using the log-rank test.The C-index,calibration curve,and receiver operator characteristic(ROC)curve were used to assess a nomogram containing clinicopathological characteristics and cuproptosis-related genes.RESULTS Pyruvate dehydrogenase A1(PDHA1)was found to be highly correlated with prognosis in univariate Cox regression analysis(hazard ratio=22.96,95%confidence interval=3.09-170.73;P=0.002).According to Kaplan-Meier survival curves,low expression of PDHA1 was associated with a better prognosis(log-rank P=0.0007).There was no significant correlation between PDHA1 expression and 22 different types of immune cells.Tumor necrosis factor superfamily member 15(TNFSF15)(P=3.2×10^(-6);r=0.37),TNFRSF14(P=8.1×10^(-8);r=0.42),H long terminal repeat-associating 2(P=6.0×10^(-8);r=0.42)and galectin 9(P=3.1×10^(-6);r=0.37)were all found to be considerably greater in the high PDHA1 expression group,according to an analysis of genes related to 47 immunological checkpoints.The low PDHA1 expression group had sig

关 键 词：Esophageal cancer Cuproptosis Pyruvate dehydrogenase A1 Overall survival NOMOGRAM 

分 类 号：R735.1[医药卫生—肿瘤]