circEIF4G2靶向miR-144-3p促进结肠癌LoVo细胞的增殖、迁移、侵袭  

CircEIF4G2 promotes proliferation,migration,and invasion of colon cancer LoVo cells by targeting miR-144-3p

作　　者：潘建柱 孙萍萍 刘丽苹 Jian-Zhu Pan;Ping-Ping Sun;Li-Ping Liu(Department of Emergency Surgery,The Fifth Central Hospital of Tianjin,Tianjin 300450,China;Department of Emergency Medicine,The Fifth Central Hospital of Tianjin,Tianjin 300450,China;Department of Neurosurgery,The Fifth Central Hospital of Tianjin,Tianjin 300450,China)

机构地区：[1]天津市第五中心医院急诊外科,天津市300450 [2]天津市第五中心医院急诊科,天津市300450 [3]天津市第五中心医院神经外科,天津市300450

出　　处：《世界华人消化杂志》2022年第23期1024-1031,共8页World Chinese Journal of Digestology

摘　　要：背景circRNA在结直肠癌中表达异常,并可通过充当miRNA海绵分子调节其靶基因表达,进而调节结直肠癌细胞生物学行为,但circEIF4G2/miR-144-3p在结直肠癌发生发展过程中的作用机制尚未明确.目的考察环状RNA(circRNA)circEIF4G2/miR-144-3p对结直肠癌LoVo细胞增殖、迁移、侵袭的影响.方法逆转录-定量聚合酶链反应检测结直肠癌组织中circEIF4G2、miR-144-3p的表达.结直肠癌LoVo细胞中转染si-NC、si-circEIF4G2、si-circEIF4G2+anti-miR-NC、si-circEIF4G2+anti-miR-144-3p,分别记为si-NC组、si-circEIF4G2组、si-circEIF4G2+anti-miR-NC组、si-circEIF4G2+anti-miR-144-3p组.利用双荧光素酶报告实验分析circEIF4G2与miR-144-3p之间的靶向结合.采用CCK-8法和克隆形成实验进行四组结直肠癌LoVo细胞的增殖抑制率、克隆形成情况测定,Transwell法检测细胞迁移、侵袭,并利用Western blotting检测上皮钙黏蛋白(E-cadherin)、神经钙黏蛋白(N-cadherin)蛋白表达.结果51例结直肠癌组织中circEIF4G2表达量比癌旁组织增加约2.38倍,miR-144-3p表达量比癌旁组织减少约0.54倍(均P<0.05).circEIF4G2靶向、调控miR-144-3p的表达.结直肠癌LoVo细胞中成功转染后,si-circEIF4G2组结直肠癌LoVo细胞的增殖抑制率、E-cadherin蛋白表达量比si-NC组增加,克隆数、迁移、侵袭数和N-cadherin蛋白表达量却比si-NC组减少(均P<0.05).si-circEIF4G2+anti-miR-144-3p组结直肠癌LoVo细胞的增殖抑制率、E-cadherin蛋白表达量低于si-circEIF4G2+anti-miR-NC组,而克隆数、迁移、侵袭数和N-cadherin蛋白表达量高于si-circEIF4G2+anti-miR-NC组(均P<0.05).结论敲减circEIF4G2通过靶向结直肠癌LoVo细胞中miR-144-3p,抑制细胞增殖、迁移和侵袭.BACKGROUND Many circular RNAs(circRNAs)are abnormally expressed in colorectal cancer,and they can regulate the expression of their target genes by acting as a miRNA sponge molecule,thereby regulating the biological behavior of colorectal cancer cells;however,the role of circEIF4G2/miR-144-3p in the occurrence and development of colorectal cancer and the underlying mechanism are not yet clear.AIM To investigate the effect of circEIF4G2/miR-144-3p on the proliferation,migration,and invasion of colorectal cancer LoVo cells.METHODS Reverse transcription-quantitative polymerase chain reaction was used to detect the expression of circEIF4G2 and miR-144-3p in colorectal cancer tissues.LoVo cells were divided into four groups and transfected with si-NC,si-circEIF4G2,si-circEIF4G2+anti-miR-NC,and si-circEIF4G2+anti-miR-144-3p,respectively.Dual luciferase reporter assay was performed to analyze the targeting relationship between circEIF4G2 and miR-144-3p.CCK-8 assay and clone formation assay were utilized to monitor the proliferation inhibition rate and clone formation in the four groups,respectively.Transwell assay was used to detect cell migration and invasion,and Western blot analysis was performed to determine E-cadherin and N-cadherin protein expression.RESULTS The expression of circEIF4G2 in 51 cases of colorectal cancer tissues increased by~2.38 times compared with tumor adjacent tissues,and the expression of miR-144-3p decreased by about 0.54 times compared with tumor adjacent tissues(P<0.05 for both).CircEIF4G2 targets and regulates the expression of miR-144-3p.The proliferation inhibition rate and E-cadherin protein expression in the si-circEIF4G2 group increased compared with those in the si-NC group,while the number of clones,migration,invasion,and the expression level of N-cadherin protein were lower than those of the si-NC group(P<0.05 for all).The proliferation inhibition rate and E-cadherin protein expression in the si-circEIF4G2+anti-miR-144-3p group were lower than those of the si-circEIF4G2+anti-miR-NC g

关 键 词：结直肠癌 LOVO细胞 增殖 迁移 侵袭 circEIF4G2 miR-144-3p 

分 类 号：R735.34[医药卫生—肿瘤]