诃子汤炮制草乌血中移行成分差异研究  

Study on difference of transitional components in blood of RadixAconiti processed by Hezi decoction

作　　者：策力木格 许良[2] 松林 图雅[4] Ce-li-mu-ge;XU Liang;Song-lin;Tu-ya(Mongolian Medical College,Inner Mongolia Minzu University,Tongliao,Inner Mongolia 028000;College of Chemistry and Materials Science,Inner Mongolia Minzu University,Tongliao,Inner Mongolia 028000;Mongolian Medical College,Inner Mongolia Medical University,Hohhot 010110,China;Research Center of Traditional Chinese Medicine,China Academy of Chinese Medical Sciences,Beijing 100700,China)

机构地区：[1]内蒙古民族大学蒙医药学院,内蒙古通辽028000 [2]内蒙古民族大学化学与材料学院,内蒙古通辽028000 [3]内蒙古医科大学蒙医药学院,内蒙古呼和浩特010110 [4]中国中医科学院中医药研发中心,北京100700

出　　处：《中国药理学通报》2022年第12期1902-1907,共6页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No 81360677,81274192,81560702);中国中医科学院科技创新工程项目(No CI2021A04209);教育部蒙医药研发工程重点实验室开放基金项目(No MDK2020021)。

摘　　要：目的分析草乌炮制前后血清中移行成分的差异,从血清药物化学的角度揭示草乌炮制机制。方法将生草乌与诃子汤炮制草乌细分0.5%羧甲基纤维素钠混悬液灌胃给药大鼠后制备含药血清,建立并分析比较空白组、生草乌组和诃子汤炮制草乌组大鼠血清UPLC特征图谱,确定草乌炮制前后血清移行成分差异。结果生草乌组含药血浆样品中检测到11个血中移行成分,鉴定出了其中6个原型成分,诃子汤炮制草乌组含药血浆样品中检测到14个血中移行成分,鉴定出了其中8个原型成分。结论草乌炮制前后血清移行成分存在显著差异,这为今后诃子汤炮制草乌机制深入研究提供实验依据。Aim To analyze the differences of transitional components in serum before and after processing of Radix Aconiti,and to reveal the processing mechanism of Radix Aconiti from the perspective of serum pharmacochemistry.Methods Radix Aconiti prepared by processing Radix Aconiti and Hezi decoction was subdivided into 0.5%sodium carboxymethylcellulose suspension,and then the drug containing serum was prepared.The UPLC characteristic maps of serum in blank group,Radix Aconiti group and Radix Aconiti prepared by processing Radix Aconiti Decoction were established,analyzed and compared,and the differences of serum migration components before and after processing were determined.Results A total of 11 blood transitional components were detected and 6 prototype components were identified in the drug containing plasma samples of raw Aconitum group.A total of 14 blood transitional components were detected and 8 prototype components were identified in the drug containing plasma samples of processed Aconitum group of Hezi decoction.Conclusions There are significant differences in serum transitional components before and after processing,which provides an experimental basis for the further study of the processing mechanism of Herba Aconiti in Hezi Decoction in the future.

关 键 词：血清药物化学 草乌 诃子 炮制机制 诃子汤炮制 UPLC-Q-TOF/MS 

分 类 号：R-332[医药卫生] R282.71R284.1R916