Wheat male-sterile 2 reduces ROS levels to inhibit anther development by deactivating ROS modulator 1  

作　　者：Jie Liu Chuan Xia Huixue Dong Pan Liu Ruizhen Yang Lichao Zhang Xu Liu Jizeng Jia Xiuying Kong Jiaqiang Sun 

机构地区：[1]National Key Facility for Crop Gene Resources and Genetic Improvement,Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081,China [2]State Key Laboratory for Agrobiotechnology and Key Laboratory of Crop Heterosis and Utilization(MOE)and Beijing Key Laboratory of Crop Genetic Improvement,China Agricultural University,Beijing 100193,China

出　　处：《Molecular Plant》2022年第9期1428-1439,共12页分子植物（英文版）

基　　金：supported by grants from the National Natural Science FoundationofChina(31871623,31991213);the National Key Research andDevelopment Program of China(2016YFD0100302);the Central Public-interest Scientific Institution Basal Research Fund(S2022D02);the Talent Program and Agricultural Science and Technology Innovation Program of CAAS.WethankLingli Zheng and Yuhong Liuforper forming the wheat transformation and planting,and Drs.Guan-Zhu Han and Yun Zhou forthe experimental assistance.

摘　　要：Ms2 is an important dominant male-sterile gene in wheat,but the biochemical function of Ms2 and the mechanism by which it causes male sterility remain elusive.Here,we report the molecular basis underlying Ms2-induced male sterility in wheat.We found that activated Ms2 specifically reduces the reactive oxygen species(ROS)signals in anthers and thereby induces termination of wheat anther development at an early stage.Furthermore,our results indicate that Ms2 is localized in mitochondria,where it physically interacts with a wheat homolog of ROS modulator 1(TaRomo1).Romo1 positively regulates the ROS levels in humans but has never been studied in plants.We found that single amino acid substitutions in the Ms2 protein that rescue the ms2 male-sterile phenotype abolish the interaction between Ms2 and TaRomo1.Significantly,Ms2 promotes the transition of TaRomo1 proteins from active monomers to inactive oligomers.Taken together,our findings unravel the molecular basis of Ms2-induced male sterility and reveal a regulatory mechanism in which ROS act as essential signals guiding the anther development program in wheat.

关 键 词：WHEAT Ms2 male sterility ROS Romo1 

分 类 号：S512.1[农业科学—作物学]