纳布啡鞘内注射对糖尿病神经痛模型大鼠行为能力及背根神经节TRPV1表达的影响  

Effects of Intrathecal Injection of Nalbuphine on Behavioral Ability and Expression of TRPV1 in Dorsal Root Ganglia in Diabetic Neuralgia Model Rats

作　　者：白静 张银福 冯媱 魏驿欢 同鑫 BAI Jing;ZHANG Yin-fu;FENG Yao;WEI Yi-huan;TONG Xin(Department of Anesthesiology,The First Affiliated Hospital of Xi'an Jiaotong University,Xi'an,Shaanxi,710089,China)

机构地区：[1]西安交通大学第一附属医院麻醉科,陕西西安710089

出　　处：《现代生物医学进展》2022年第20期3823-3827,共5页Progress in Modern Biomedicine

基　　金：陕西省自然科学基础研究计划项目(2017JQ8027)。

摘　　要：目的:探讨纳布啡鞘内注射对糖尿病神经痛(diabetic neuropathic pain,DNP)模型大鼠行为能力及背根神经节瞬时受体电位V1(transient receptor potential V1,TRPV1)表达的影响。方法:将糖尿病神经痛模型大鼠(n=48)随机平方为三组-模型组、纳布啡1组与纳布啡2组,每组16只。纳布啡1组与纳布啡2组分别给予纳布啡鞘内注射0.5μg/10μL与1.0μg/10μL,模型组给予注射等剂量的0.9%氯化钠溶液,每天1次。分别于治疗第7 d、第14 d,采用血糖仪测定与记录空腹血糖(fasting blood glucose,FBG)水平并进行机械痛阈检测;治疗第14 d、第28 d,采用酶联免疫法检测血清IL-6与TNF-α含量,采用免疫印迹法检测背根神经节TRPV1蛋白的相对表达。结果:模型组、纳布啡1组与纳布啡2组治疗第7 d、第14 d的空腹血糖水平都高于20.00 mmol/L,组内与组间对比差异不具有统计学意义(P>0.05)。纳布啡1组与纳布啡2组治疗第7 d、第14 d的机械痛阈高于模型组(P<0.05),也高于治疗前(P<0.05),纳布啡2组与纳布啡1组差异具有统计学意义(P<0.05)。纳布啡1组与纳布啡2组治疗第14 d、第28 d的血清白细胞介素(Interleukin,IL)-6与肿瘤坏死因子(Tumor necrosis factor,TNF)-α含量明显低于模型组(P<0.05),纳布啡2组明显低于纳布啡1组(P<0.05)。纳布啡1组与纳布啡2组治疗第14 d、第28 d的背根神经节TRPV1相对表达水平明显低于模型组(P<0.05),纳布啡2组明显低于纳布啡1组(P<0.05)。结论:纳布啡鞘内注射在糖尿病神经痛模型大鼠的应用能改善行为能力,抑制背根神经节TRPV1的表达,还可抑制血清IL-6与TNF-α的释放,从而发挥镇痛治疗效用。Objective:To investigate the effects of intrathecal injection of nalbuphine on the behavioral ability and the expression of transient receptor potential V1(TRPV1)in the dorsal root ganglion of diabetic neuralgia model rats.Methods:The diabetic neuralgia model rats(n=48)were randomly divided into three groups-model group,nalbuphine group 1 and nalbuphine group 2,with 16 rats in each groups.The nalbuphine group 1 and the nalbuphine group 2 were given intrathecal injection of 0.5μg/10μL and 1.0μg/10μL of nalbuphine respectively,and the model group were given an equal dose of 0.9%sodium chloride solution,once a day,On the 7th and 14th days of treatment,the fasting blood glucose(FBG)level was measured and recorded with a blood glucose meter,and the mechanical pain threshold was detected;On the 14th and 28th days of treatment,the serum levels of IL-6 and TNF-αwere detected by enzyme-linked immunosorbent assay,and the relative expression of TRPV1 protein in the dorsal root ganglia was detected by western blotting.Results:The fasting blood glucose levels of the model group,nalbuphine group 1 and nalbuphine group 2 were all higher than 20.00 mmol/L on the 7th and 14th days of treatment,and the differences between the groups were not statistically significant(P>0.05).The mechanical pain threshold of nalbuphine group 1 and nalbuphine group 2 on the 7th and 14 th days of treatment were higher than that of the model group(P<0.05),and were also higher than that before treatment(P<0.05).The difference between the nalbuphine 2 group and the nalbuphine 1 group was statistically significant(P<0.05).The levels of serum interleukin(IL)-6 and tumor necrosis factor(TNF)-αin the nalbuphine 1 group and the nalbuphine 2 group on the 14 th and 28 th days of treatment were significantly lower than those in the model group(P<0.05),the nalbuphine 2 group were significantly lower than the nalbuphine 1 group(P<0.05).The relative expression levels of TRPV1 in the dorsal root ganglia of the nalbuphine group 1 and nalbuphine group 2 on the

关 键 词：纳布啡 鞘内注射 糖尿病神经痛 大鼠 背根神经节 瞬时受体电位V1 

分 类 号：R-33[医药卫生] R587.2R614