条斑紫菜R-藻红蛋白的分离纯化及稳定性研究  

Isolation, Purification and Stability of Phycoerythrin from Porphyra yezoensis Ueda

作　　者：董硕 姜璐瑶 聂岩 翟婧 张冉 李文军[2] 唐志红[1] Dong Shuo;Jiang Luyao;Nie Yan;Zhai Jing;Zhang Ran;Li Wenjun;Tang Zhihong(College of Life Science,Yantai University,Yantai,Shandong 264005,China;Yantai Institute of Coastal Zone Research,Chinese Academy of Sciences,Yantai,Shandong 264003,China)

机构地区：[1]烟台大学生命科学学院,山东烟台264005 [2]中国科学院烟台海岸带研究所,山东烟台264003

出　　处：《绿色科技》2022年第22期210-214,共5页Journal of Green Science and Technology

基　　金：山东省自然科学基金联合基金项目(编号:ZR2021LLZ003);烟台大学研究生科技创新基金(编号:GGIFYTU2224)。

摘　　要：以条斑紫菜为原料,采用硫酸铵盐析结合Phenyl-sepharose FF疏水层析技术分离纯化R-藻红蛋白,并对其稳定性进行了研究。结果表明:采用硫酸铵分级沉淀,R-藻红蛋白纯度(A_(565)/A_(280))可提高到0.83,回收率为75.36%;再经疏水层析纯化后,R-藻红蛋白的纯度(A_(565)/A_(280))为5.47,达到试剂级,回收率为56.92%。纯化藻红蛋白的最大特征吸收峰和荧光发射峰分别位于565 nm、576 nm处,主要的二级结构元件形式为α-螺旋,分子中含有α、β和γ三个亚基。R-藻红蛋白在低于60℃、pH值5~7条件下能保持相对稳定。In this study,R-phycoerythrin was isolated and purified from Porphyra yezoensis Ueda by ammonium sulfate salting out combined with Phenyl sepharose FF hydrophobic chromatography,and its stability was studied.The results showed that the purity of R-phycoerythrin(A_(565)/A_(280))could be increased to 0.83 and the recovery was 75.36%by ammonium sulfate precipitation;After purification by hydrophobic chromatography,the purity of R-phycoerythrin(A_(565)/A_(280))was 5.47,reaching reagent level,and the recovery was 56.92%.The maximum characteristic absorption peak and fluorescence emission peak of purified R-phycoerythrin are located at 565 nm and 576 nm,respectively.The main secondary structural elements areα-Helix,containingα,βandγthree subunits in the molecule.R-Phycoerythrin is relatively stable under the conditions of below 60℃and pH5~7.

关 键 词：条斑紫菜 R-藻红蛋白 分离纯化 稳定性 

分 类 号：TS202.3[轻工技术与工程—食品科学]