敲低Prdx4抑制三阴性乳腺癌MDA-MB-231细胞侵袭和促进其凋亡的机制  

The mechanism for Prdx4 knockdown to inhibit invasion and promote apoptosis of triple-negative breast cancer MDA-MB-231 cells

作　　者：魏静[1] 王石健[1] 杨赛成[1] 夏修远[1] 周雪峰 王金明[1] WEI Jing;WANG Shijian;YANG Saicheng(Department of Pharmacy,Taizhou Municipal Hospital,Taizhou 318000,CHINA)

机构地区：[1]台州市立医院药剂科,浙江318000

出　　处：《江苏医药》2022年第10期986-989,共4页Jiangsu Medical Journal

基　　金：台州市科技计划项目(20ywb53)。

摘　　要：目的探讨敲低过氧化物还原酶4(Prdx4)抑制三阴性乳腺癌MDA-MB-231细胞侵袭和促进其凋亡的机制。方法用转染试剂Lipofectamine 2000将对照小干扰RNA(siRNA)和Prdx4 siRNA转染MDA-MB-231细胞分为si-NC组和si-Prdx4组。采用流式细胞术检测细胞凋亡,细胞划痕实验检测细胞迁移,Transwell实验检测细胞侵袭数目,Western blot法检测Prdx4、MMP-2、MMP-9、N-钙黏蛋白、E-钙黏蛋白、波形蛋白和Snail蛋白的蛋白表达。结果与si-NC组相比,si-Prdx4组Prdx4蛋白表达下降,且细胞迁移率降低,细胞侵袭数目减少,MMP-2、MMP-9、N-钙黏蛋白、波形蛋白和Snail蛋白表达下降,E-钙黏蛋白表达上升(P<0.05)。结论敲低Prdx4后能够抑制三阴性乳腺癌MDA-MB-231细胞侵袭和促进细胞凋亡。Objective To investigate the mechanism for the knockdown of peroxiredoxin 4(Prdx4)to inhibit the invasion and promote the apoptosis of the triple-negative breast cancer MDA-MB-231 cells.Methods MDA-MB-231 cells were transfected with control interfering RNA(siRNA)and Prdx4 siRNA using transfection reagent Lipofectamine 2000,which then were divided into two groups of si-NC and si-Prdx4.Cell apoptosis was detected by flow cytometry.Cell migration was detected by cell scratch assay.The number of cell invasion was detected by Transwell assay.Western blot method was used to detect the protein levels of Prdx4,MMP-2,MMP-9,N-cadherin,E-cadherin,vimentin and Snail.Results Compared with group si-NC,the expression of Prdx4 protein,cell migration rate and number of invasive cells were decreased in group si-Prdx4(P<0.05).The protein expressions of MMP-2,MMP-9,N-cadherin,vimentin and Snail were decreased and E-cadherin was increased in group si-Prdx4 than those in group si-NC(P<0.05).ConclusionKnockdown of Prdx4 can inhibit the invasion and promote the apoptosis of the triple-negative breast cancer MDA-MB-231 cells.

关 键 词：过氧化物还原酶4 三阴性乳腺癌 侵袭 凋亡 

分 类 号：R737[医药卫生—肿瘤]