红曲橙色素与红曲黄色素定量分析方法改进  

Improvement of Quantitative Analysis of Monascus Orange Pigments and Monascus Yellow Pigments

作　　者：杨曼红 郭佳[1] 杨露 朱效珍 王晶[1] 陈勉华[1] YANG Man-hong;GUO Jia;YANG Lu;ZHU Xiao-zhen;WANG Jing;CHEN Mian-hua(College of Food Science and Engineering,Tianjin University of Science&Technology,Tianjin 300457,China)

机构地区：[1]天津科技大学食品科学与工程学院,天津300457

出　　处：《食品研究与开发》2022年第24期175-180,共6页Food Research and Development

基　　金：国家自然科学基金面上项目(31571820)。

摘　　要：该文研究红曲橙色素与红曲黄色素的定量分析方法。通过分光光度法、高效液相色谱(high performance liquid chromatography,HPLC)法和柱层析法,筛选红曲橙色素和红曲黄色素的提取溶剂。结果表明,石油醚∶乙酸乙酯(体积比2∶1)与石油醚∶二氯甲烷(体积比2∶1)明显提高红曲橙色素与红曲黄色素的提取率。该文建立高效液相色谱法快速定量红曲橙色素与红曲黄色素的分析方法,在10μg/mL~500μg/mL范围内线性关系良好(R^(2)>0.997)。This study aims to improve the quantitative analysis of Monascus orange pigments and Monascus yellow pigments.The extraction solvents of Monascus orange pigments and Monascus yellow pigments were screened by spectrophotometry,high performance liquid chromatography(HPLC),and column chromatography.The results showed that mixture of petroleum ether and ethyl acetate(volume ratio=2∶1)and mixture of petroleum ether and dichloromethane(volume ratio=2∶1)significantly improved the extraction rate of Monascus orange pigments and Monascus yellow pigments.A rapid and accurate HPLC method was established,and the pigments showed good linear relationship in the range of 10μg/mL-500μg/mL(R^(2)>0.997).

关 键 词：定量分析 高效液相色谱 红曲橙色素 红曲黄色素 提取溶剂 

分 类 号：TS264.4[轻工技术与工程—发酵工程]