珠江广州段浮游真核微生物分布及其法医学意义  

Distribution and Forensic Relevancy of Eukaryotic Microplanktons in Guangzhou Section of the Pearl River

作　　者：苏秦 余仲昊 徐曲毅[1] 吴伟斌 李欢 杜蔚安 赵建[1] 陈玲 刘宏[1] 刘超[1,2] SU Qin;YU Zhonghao;XU Quyi;WU Weibin;LI Huan;DU Weian;ZHAO Jian;CHEN Ling;LIU Hong;LIU Chao(Forensic Pathological Key Laboratory(Guangzhou)of Ministry of Public Security&Guangzhou Institute of Forensic Science,Guangzhou 510442,China;School of Forensic Science,Southern Medical University,Guangzhou 510515,China)

机构地区：[1]广州市刑事科学技术研究所,公安部法医病理学重点实验室,广州510442 [2]南方医科大学法医学院,广州510515

出　　处：《刑事技术》2022年第6期593-598,共6页Forensic Science and Technology

基　　金：广州市科技计划(2019030001、2019030012、202102080308);公安部科技强警基础工作专项(2020GABJC38)。

摘　　要：通过对珠江水域(广州段)水样进行18S rDNA测序分析,探究同一水域不同地点的硅藻及其他浮游真核微生物的分布和差异,以期为溺死诊断和溺水地点的推断提供参考依据。在珠江水域(广州段)随机等距离选取4个取水点采集水样,采用18S rDNA测序对各取水点的硅藻及其他浮游真核微生物进行定性和定量分析。珠江不同取水点包括硅藻在内的浮游真核微生物的种类数量(OTU数)平均为483.73±39.06个,但不同取水点中的数量具有显著差异。同时,不同取水点的微型浮游生物组成比例也存在显著差异。取水点W1与W4中存在特异的硅藻属:W1为脆杆藻属;W4为圆筛藻属、细柱藻属和泥生藻属。种水平上的分析显示,所有取水点均具有特异物种,而以W4中为最多,达98种。珠江水域(广州段)不同地点的浮游真核微生物群落分布及其差异可为死因和溺水地点的推断提供参考,并可用于建立珠江水域的浮游真核微生物数据库。同时,珠江水域不同地点存在的特异物种,也可为溺水地点的推断提供新指向。In order to search for the distribution and differences of diatoms and other eukaryotic microplanktons at discrepant locations in the same water area so as to provide a basis for both diagnosis about drowning and inference of drowning site,we randomly selected 4 equidistant water intake points(W1,W2,W3 and W4)in the Pearl River(Guangzhou section)to collect water samples having 18S rDNA sequenced to analyze qualitatively and quantitatively those diatoms and other eukaryotic microplanktons at each water-collecting site.It was found that the average number of species of eukaryotic microplanktons including diatoms was 483.73±39.06,and the number of species in the discrepant water sampling locations was remarkably different.Meanwhile,there were signifi cant differences among the eukaryotic microplanktons from discrepant water sampling locations.Specifi c diatoms were found in W1 and W4,showing Fragilaria in W1 and in W4 as Coscinodiscus,Cylindrotheca and Luticola.Moreover,Analysis revealed that all the sampling locations had their specifi c species at the species level,with W4 harboring the most amount of specifi c species totalling to 98 ones.These fi ndings suggest that the distribution and differences of the eukaryotic microplanktonic communities at different locations in the Pearl River(Guangzhou section)can be used for the inference of related death cause and drowning site.Moreover,the specifi c species at different locations in the Pearl River can provide a new insight into inference of drowning site.The eukaryotic microplanktonic communities distribute their involving species discrepantly at different locations in Guangzhou section of the Pearl River,enabling a reference and inference about related death cause and drowning site,and also establishment of the database of eukaryotic microplanktons in the Pearl River.

关 键 词：法医物证学 法医病理学 溺水地点 18S核糖体脱氧核糖核酸 真核微浮游生物 

分 类 号：DF795.2[医药卫生—法医学] DF795.1[政治法律—诉讼法学]