干扰LINC00313可促进miR-302的表达影响宫颈癌细胞增殖及凋亡  

Interference with LINC00313 could promote the expression of miR-302 and affect the proliferation and apoptosis of cervical cancer cells

作　　者：王红红[1] 刘静 骆硕 李克用 WANG Honghong;LIU Jing;LUO Shuo;LI Keyong(Baoding Maternal and Child Health Hospital,Baoding,Hebei 071000,China;the Eighth People’s Hospital of Jinan,Jinan,Shandong 271104,China)

机构地区：[1]保定市妇幼保健院,河北保定071000 [2]山东济南第八人民医院,山东济南271104

出　　处：《中国优生与遗传杂志》2022年第10期1736-1741,共6页Chinese Journal of Birth Health & Heredity

摘　　要：目的 探讨lncRNALINC00313对宫颈癌细胞增殖、凋亡的影响及其对miR-302的调控作用。方法 采用qRT-PCR法检测宫颈癌组织、癌旁组织中LINC00313的表达水平;体外培养人宫颈癌细胞SiHa,分为si-NC组(转染si-NC)、si-LINC00313组(转染si-LINC00313)、si-LINC00313+anti-miR-302组(共转染si-LINC00313、anti-miR-302)、si-LINC00313+anti-miR-NC组(共转染si-LINC00313、anti-miR-NC)。qRT-PCR检测LINC00313、miR-302表达水平;MTT实验检测细胞增殖;流式细胞仪检测细胞周期及凋亡率;双荧光素酶报告实验检测LINC00313、miR-302的靶向关系;Western blot法检测Bax、Bcl-2蛋白水平。结果 与癌旁组织比较,宫颈癌组织LINC00313的表达水平显著升高(P<0.05);转染si-LINC00313可明显降低OD值、S期细胞比例及Bcl-2蛋白水平(P<0.05),提高G0-G1期细胞比例、凋亡率及Bax蛋白水平(P<0.05);双荧光素酶报告实验证实LINC00313可靶向结合miR-302;抑制miR-302表达可逆转干扰LINC00313对SiHa细胞增殖及凋亡的影响。结论 干扰LINC00313可通过上调miR-302抑制宫颈癌细胞增殖和诱导细胞凋亡。Objective To explore the effect of LncRNA LINC00313 on the proliferation and apoptosis of cervical cancer cells and its regulatory effect on miR-302. Methods Detection of the expression level of LINC00313 in cervical cancer tissue and adjacent tissue by qRT-PCR. In vitro culture of human cervical cancer cell SiHa, Divided into si-NC group(transfected with si-NC), si-LINC00313 group(transfected with si-LINC00313), and si-LINC00313+anti-miR-NC group(co-transfected with si-LINC00313 and anti-miR-NC), si-LINC00313+anti-miR-302 group(co-transfected with si-LINC00313and anti-miR-302). The expression levels of LINC00313 and miR-302 were detected by qRT-PCR;cell proliferation was detected by MTT assay. Cell cycle and apoptosis rate were detected by flow cytometry. The dual luciferase reporter experiment detects the targeting relationship of LINC00313 and miR-302. Western blot method was used to detect the protein levels of Bax and Bcl-2. Results Compared with adjacent tissues, the expression level of LINC00313 in cervical cancer tissue was significantly increased(P<0.05). Transfection of si-LINC00313 could significantly reduce the OD value, the proportion of cells in S phase and the protein level of Bcl-2(P<0.05), and increase the proportion of cells in the G0-G1 phase, the rate of apoptosis and the protein level of Bax(P<0.05). LINC00313 could target miR-302. Inhibiting the expression of miR-302 could reverse the effect of interfering with LINC00313 on the proliferation and apoptosis of SiHa cells. Conclusion Interference with LINC00313 inhibits cervical cancer cell proliferation and induces apoptosis by upregulating miR-302.

关 键 词：lncRNA LINC00313 miR-302 宫颈癌 增殖 凋亡 

分 类 号：R737.33[医药卫生—肿瘤]