TTLL12在鼻咽癌中的表达及临床意义  

Expression and clinical significance of TTLL12 in nasopharyngeal carcinoma

作　　者：吴春成 连雅晨 邓世山[2] 杨洪斌[1] WU Chuncheng;LIAN Yachen;DENG Shishan;YANG Hongbin(Department of Otolaryngology Head and Neck Surgery,Affiliated Hospital of North Sichuan Medical College,Sichuan,Nanchong 637000,China;Department of Anatomy,North Sichuan Medical College,Sichuan,Nanchong637000,China)

机构地区：[1]川北医学院附属医院耳鼻咽喉头颈外科,四川南充637000 [2]川北医学院解剖教研室,四川南充637000

出　　处：《中国现代医生》2022年第34期42-45,61,共5页China Modern Doctor

基　　金：校级科研发展计划项目(CBY21-QA25)。

摘　　要：目的探讨鼻咽癌患者中微管蛋白酪氨酸连接酶类似物12(tubulin tyrosine ligase like 12,TTLL12)的表达及临床意义。方法利用免疫组织化学法研究TTLL12在鼻咽慢性炎症组织和鼻咽癌组织中的表达,分析其在不同鼻咽组织中的表达差异及与鼻咽癌患者临床特征之间的相关性。从GEO数据库中筛选合适的鼻咽癌基因芯片数据集GSE102349,分析不同临床分期中TTLL12基因在转录水平上的表达差异、TTLL12与鼻咽癌患者无进展生存的相关性及可能参与的通路。结果免疫组织化学结果显示,与鼻咽慢性炎症组织相比,TTLL12蛋白在鼻咽癌组织中表达明显上调(P<0.001)。TTLL12高表达鼻咽癌患者的T_(3)-T_(4)、N_(2)-N_(3)、Ⅲ~Ⅳ期的占比显著高于TTLL12低表达患者(P<0.05)。TTLL12高表达患者的总生存显著低于低表达患者(P=0.027)。TTLL12高表达和N分期均是影响鼻咽癌患者预后的独立危险因素(P<0.05)。在基因芯片数据集中,TTLL12 mRNA在鼻咽癌组织中的表达与临床分期呈正相关(P=0.033),TTLL12高表达的鼻咽癌患者无进展生存率显著低于TTLL12低表达患者(P=0.041)。KEGG富集分析显示TTLL12高表达基因样本主要富集在趋化因子信号通路,与人类T细胞白血病病毒1型感染及EB病毒感染密切相关。结论TTLL12表达上调作为不利因素可促进鼻咽癌的发生发展。Objective To explore the expression and clinical significance of tubulin tyrosine ligase like 12(TTLL12) in patients with nasopharyngeal carcinoma. Methods Immunohistochemistry was used to study the expression of TTLL12 in nasopharyngeal chronic inflammatory tissues and nasopharyngeal carcinoma tissues, and to analyze the difference of TTLL12 expression in different nasopharyngeal tissues and its correlation with clinical features of patients with nasopharyngeal carcinoma. A suitable nasopharyngeal carcinoma microarray dataset GSE102349 was screened from the GEO database to analyze the expression difference of TTLL12 gene at the transcription level in different clinical stages,the correlation between TTLL12 and progression free survival of nasopharyngeal carcinoma patients, and the possible pathways involved. Results Immunohistochemical results showed that TTLL12 protein expression was significantly up-regulated in nasopharyngeal carcinoma tissues compared with nasopharyngeal chronic inflammatory tissues(P<0.001). The proportion of T_(3)-T_(4),N_(2)-N_(3), Ⅲ-Ⅳ in patients with high expression of TTLL12 was significantly higher than that in patients with low expression of TTLL12(P<0.05). Overall survival in patients with high TTLL12 expression was significantly lower than that in patients with low expression(P=0.027). Both the high expression of TTLL12 and N stage were independent risk factors for the prognosis of nasopharyngeal carcinoma patients(P<0.05). In the microarray data set, the expression of TTLL12 m RNA in nasopharyngeal carcinoma tissues was positively correlated with the clinical stage(P=0.033), and the progression free survival of patients with high expression of TTLL12 was significantly lower than that of patients with low expression of TTLL12(P=0.041). KEGG enrichment analysis showed that TTLL12highly expressed gene samples were mainly enriched in chemokine signaling pathway, which was closely related to human T-cell leukemia virus type 1 infection and Epstein-Barr virus infection. Conclusion T

关 键 词：鼻咽肿瘤 微管蛋白酪氨酸连接酶类似物12 免疫组织化学 预后 GEO数据库 

分 类 号：R739.6[医药卫生—肿瘤]