UGT2B15在胃癌细胞中的表达及影响  

Expression of UGT2B15 in gastric cancer and its effect on gastric cancer cells

作　　者：陈选民[1] 周伟伟[1] 谭芳芳 宾玉玲 胡光胜[1] Chen Xuanmin;Zhou Weiwei;Tan Fangfang;Bin Yuling;Hu Guangsheng(Department of Gastroenterology,the First Affiliated Hospital of South China of University,Hengyang 421001,Hunan,China)

机构地区：[1]南华大学附属第一医院消化内科,湖南衡阳421001

出　　处：《实用检验医师杂志》2022年第3期316-320,共5页Chinese Journal of Clinical Pathologist

基　　金：湖南省教育厅科学研究项目(18C0459)。

摘　　要：目的探讨葡糖醛酸转移酶2家族B15(UGT2B15)在胃癌细胞中的表达及影响。方法回顾并分析2015年1月—2019年12月南华大学附属第一医院收治的226份胃腺癌患者的肿瘤组织和对应癌旁组织资料。通过免疫组化和实时荧光定量聚合酶链反应(qRT-PCR)检测UGT2B15的表达,分析其与患者临床病理特征和预后的关系;采用小干扰RNA(siRNA)抑制UGT2B15在胃癌细胞系AGS和MGC-803的表达后,以CCK-8、流式细胞术及Transwell实验检测UGT2B15对胃癌细胞增殖、凋亡和侵袭能力的影响;采用免疫印迹法(Western blotting)和qRT-PCR检测抑制UGT2B15表达后的FOXA1表达水平。结果胃癌组织中UGT2B15和FOXA1的表达明显高于癌旁组织,且UGT2B15在胃癌中的表达与胃癌肿瘤浸润深度、脉管侵犯以及TNM分期密切相关。UGT2B15阳性表达组患者的疾病特定生存率(DSS)和总体生存率(OS)均明显低于阴性表达组。siRNA抑制AGS和MGC-803细胞中UGT2B15的表达后,抑制组的细胞增殖明显低于对照组(AGS:0.67±0.25比1.75±0.43,MGC-803:0.82±0.33比1.86±0.47,均P<0.05),吸光度值(A)明显低于对照组(AGS:472.0±36.5比700.3±82.2,MGC-803:487.2±18.2比638.5±21.3,均P<0.05),细胞凋亡率明显升高〔(17.2±6.4)%比(8.7±3.4)%,P<0.05〕;siRNA抑制胃癌细胞中UGT2B15表达后,FOXA1蛋白和RNA表达均明显降低(蛋白表达:0.091±0.018比0.045±0.012,RNA表达:AGS:1.000比0.582±0.124,MGC-803:1.000比0.724±0.157,均P<0.05)。结论UGT2B15在胃癌组织中表达升高,且其表达水平与胃癌肿瘤浸润深度、脉管侵犯、TNM分期以及不良预后密切相关;UGT2B15可能参与胃癌细胞增殖、侵袭以及凋亡;抑制胃癌细胞中UGT2B15的表达后可降低FOXA1表达。Objective To investigate the expression of glucuronyltransferase 2 family B15(UGT2B15)in gastric cancer cells and its effect.Methods From January 2015 to December 2019,the tumor tissue and corresponding adjacent tissue data of 100 patients with gastric adenocarcinoma in the First Affiliated Hospital of South China University were retrospectively analyzed.The expression of UGT2B15 was detected by immunohistochemistry and real-time quantitative polymerase chain reaction(qRT-PCR),and its relationship with clinicopathological characteristics and prognosis was analyzed.Small interfering RNA(siRNA)was used to inhibit the expression of UGT2B15 in gastric cancer cell lines AGS and MGC-803,flow cytometry and Transwell experiment were used to detect the effects on proliferation,apoptosis and invasion of gastric cancer cells.Western blotting and qRT-PCR were used to detect the expression of FOXA1 after inhibiting the expression of UGT2B15.Results The expression of UGT2B15 and FOXA1 in gastric cancer tissues was higher than that in adjacent tissues,and the expression of UGT2B15 in gastric cancer was significantly correlated with the depth of tumor invasion,vascular invasion and TNM stage.The disease-specific survival(DSS)and overall survival(OS)of patients in UGT2B15 positive expression group were lower than those in negative expression group.After siRNA inhibited the expression of UGT2B15 in AGS and MGC-803 cells,the cell proliferation in inhibition group was lower than that in control group(AGS:0.67±0.25 vs.1.75±0.43,MGC-803:0.82±0.33 vs.1.86±0.47,both P<0.05),the absorbance value(A)was significantly lower than that in the control group(AGS:472.0±36.5 vs.700.3±82.2,MGC-803:487.2±18.2 vs.638.5±21.3,all P<0.05),and the poptosis rate increased significantly[(17.2±6.4)%vs.(8.7±3.4)%,P<0.05].After siRNA inhibited the expression of UGT2B15 in gastric cancer cells,the protein and RNA expression of FOXA1 decreased significantly(protein expression:0.091±0.018 vs.0.045±0.012,RNA expression:AGS:1.000 vs.0.582±0.124,MGC-8

关 键 词：胃癌 UGT2B15 不良预后 

分 类 号：R735.2[医药卫生—肿瘤]