蓝花黄芩内生真菌Aspergillus terreus HQ100X-1次级代谢产物研究  

Study on the Secondary Metabolites of the Endophytic Fungus Aspergillus terreus HQ100X-1 in Scutellaria formosana

作　　者：黄丹 农旭华 杨建妮 李琛 韩长日 陈光英[1,2] 宋小平[1,2] 孙振范 惠阳[1,2] 陈文豪[1,2] Dan Huang;Xuhua Nong;Jianni Yang;Chen Li;Changri Han;Guangying Chen;Xiaoping Song;Zhenfan Sun;Yang Hui;Wenhao Chen(Key Laboratory of Tropical Medicinal Resource Chemistry of Ministry of Education,College of Chemistry and Chemical Engineering,Hainan Normal University,Haikou 571158;Key Laboratory of Tropical Medicinal Plant Chemistry of Hainan Province,College of Chemistry and Chemical Engineering,Hainan Normal University,Haikou 571158;Key Laboratory of Medicinal and Edible Plants Resources of Hainan Province,Hainan Vocational University of Science and Technology,Haikou 571126)

机构地区：[1]海南师范大学化学与化工学院热带药用资源化学教育部重点实验室,海口571158 [2]海南师范大学化学与化工学院热带药用植物化学海南省重点实验室,海口571158 [3]海南科技职业大学药食同源植物资源海南省重点实验室,海口571126

出　　处：《有机化学》2022年第9期2961-2966,共6页Chinese Journal of Organic Chemistry

基　　金：国家自然科学基金(No.22167013);海南省重点研发项目(No.ZDYF2021SHFZ221);海南省自然科学基金(No.221RC541);海南省教育厅项目(No.Hnky2021ZD-10);海南省研究生创新科研课题(No.Hys2021-72);大学生创新创业训练计划(Nos.202111658002,202111658003)资助项目.

摘　　要：从蓝花黄芩内生真菌土曲霉(Aspergillusterreus)HQ100X-1发酵产物中分离得到2个新化合物[terrustone(1)和asperteretone G (2)], 10个已知化合物(3~12), terrustone (1)为具有4个连续手性中心的三羟基环戊酮类化合物,它们的结构以及绝对构型是通过1D/2D NMR结合电子圆二色谱(ECD)计算来确定.抗菌实验表明,化合物rbrolide R (4)对金黄色葡萄球菌有一定的抑菌活性,其最低抑菌浓度(MIC)值为2.5μg/mL.Two new compounds, terrustone(1) and asperteretone G(2) together with ten known compounds were isolated from Scutellaria formosana endophytic fungus Aspergillus terreus HQ100X-1, terrustone(1) is a trihydroxycyclopentanone with four consecutive chiral centers. Their structures were determined by 1D/2D NMR data along with electrostatic circular dichroism(ECD) calculation for configurational assignment. Compound rbrolide R(4) showed antibacterial activity against Staphylococcus aureus with minimum inhibitory concentration(MIC) value of 2.5 μg/m L.

关 键 词：蓝花黄芩 土曲霉(Aspergillus terreus) 抗菌活性 

分 类 号：TQ920.1[轻工技术与工程—发酵工程]