餐具中残留十二烷基苯磺酸免疫层析检测卡研究  

Development of Immunochromatographic Assay for Screening of Benzenesulfonic Acid in Tableware

作　　者：唐桂红 郭杰标[2] 陈华龙 江伟伶 姜丽君 吴帆敏 TANG Gui-hong;GUO Jie-biao;CHEN Hua-long;JIANG Wei-ling;JIANG Li-jun;WU Fan-min(Shaoguan Institute for Food and Drug Control,Guangdong Shaoguan 512026;Shaoguan College,Henry Fok Food College,Guangdong Shaoguan 512005,China)

机构地区：[1]韶关市食品药品检验所,广东韶关512026 [2]韶关学院英东食品学院,广东韶关512005

出　　处：《广州化工》2022年第21期142-145,共4页GuangZhou Chemical Industry

基　　金：韶关市科技计划项目(No:2019sn118);广东省自然科学基金项目(No:2018A0303070010);广东省市场监督管理局2022年度科技项目(No:2022CS09)。

摘　　要：建立筛查苯磺酸表面活性剂的荧光免疫检测卡;以对磺基苯甲酸合成免疫抗原,免疫家兔获得针对苯磺酸基团的多克隆抗体,纯化后标记量子点荧光微球;以对氨基苯磺酸合成检测抗原,构建荧光免疫层析卡(FIAC)。在酶免疫分析中对十二烷基苯磺酸钠的IC_(50)值为55.2 ng/mL,优化的FIAC对十二烷基苯磺酸钠的检测限为100 ng/mL。本方法灵敏、特异、简便,是筛查餐具中残留烷基苯磺酸表面活性剂的有效工具。Aiming to develop a fluorescent immunochromatographic assay(FICA)for screening residual surfactants of benzenesulfonic acid,P-sulfobenzoic acid was employed to synthesize immune antigen,rabbits were immunized to obtain polyclonal antibodies against benzenesulfonic acid group,quantum dots(150 nm)were labeled with purified antibodies for fluorescent probe,P-aminobenzenesulfonic acid was applied for preparation of testing antigen for immunossays.The IC_(50) value of sodium dodecylbenzene sulfonate in the ELISA system was 55.2 ng/mL,and the detection limit of sodium dodecylbenzene sulfonate in the optimized FIAC strip was 100 ng/mL.The current FIAC is an effective tool for screening residual surfactants of benzenesulfonic acid in tableware,due to being sensitive,specific,and simple.

关 键 词：餐具卫生 苯磺酸类表面活性剂 荧光免疫层析检测 人工抗原 免疫微球 

分 类 号：R392.11[医药卫生—免疫学] R446.61[医药卫生—基础医学]