食管癌术后肺部感染血浆TNF基因微卫星多态性及与其预后的关系  

Relationship Between Plasma TNF Gene Microsatellite Polymorphism and Prognosis in Postoperative Pulmonary Infection of Esophageal Carcinoma

作　　者：师智勇 张振世 刘圣武 SHI Zhiyong;ZHANG Zhenshi;LIU Shengwu(General Hospital of China Pingmei Shenma Group,Pingdingshan,467000)

机构地区：[1]中国平煤神马集团总医院,467000

出　　处：《实用癌症杂志》2022年第12期1990-1993,共4页The Practical Journal of Cancer

摘　　要：目的探讨食管癌术后肺部感染患者血浆肿瘤坏死因子(TNF)基因微卫星多态性及与其预后的关系。方法将30例食管癌术后继发肺部感染患者设为观察组,同期食管癌术后未发生感染的120例患者设为对照组,并依据观察组患者预后情况(住院28 d是否死亡)分为生存组(n=25)与死亡组(n=5)。运用聚合酶链式反应行DNA扩增,以聚丙烯酰胺凝胶电泳-银染技术施以微卫星分型。比较观察组与对照组、生存组与死亡组TNFc微卫星基因型与等位基因分布,并进行预后分析。结果观察组携带TNFc1/1基因型与TNFc1等位基因比例高于对照组,差异有统计学意义(P<0.05);肺部感染患者中死亡组携带TNFc1/1基因型与TNFc1等位基因比例高于生存组,差异有统计学意义(P<0.05);多因素分析显示:血液高凝状态、低蛋白血症以及携带TNFc1/1基因型为造成患者预后不良的危险因素(P<0.05)。结论TNF基因微卫星多态性与食管癌术后肺部感染及预后密切相关,TNFc1/1基因型会增加食管癌患者易感性,导致不良预后,临床需予以高度重视。Objective To investigate the relationship between the microsatellite polymorphism of the plasma tumor necrosis factor(TNF)gene and its prognosis in patients with lung infection after oesophageal cancer.Methods 30 patients with secondary lung infection after esophageal cancer were assigned as the observation group,and 120 patients without infection after esophageal cancer were assigned as the control group:survival(n=25)and (n=5)according to the prognosis(28d death)(n=5).DNA amplification was performed by polymerase chain reaction,and microsatellite typing was performed by polyacrylamide gel electrophoresis-silver dye technology.The TNFc microsatellite genotype and allele distribution between the observation group and the control group,and the survival and dead groups were compared,and the prognostic analysis was performed.Results The proportion of TNFc1/1 and TNFc1 was higher than the control group(P<0.05)and TNFc1(P<0.05);Multivariate analysis showed that blood hypercoagulation,hypoproteinemia,and TNFc1/1 genotype were risk factors for poor prognosis(P<0.05).Conclusion The TNF gene microsatellite polymorphism is closely related to lung infection and prognosis after esophageal cancer surgery.The TNFc1/1 genotype will increase the susceptibility of esophageal cancer patients,leading to poor prognosis,requiring great clinical attention.

关 键 词：食管癌 肺部感染 肿瘤坏死因子 基因微卫星多态性 预后 

分 类 号：R735.1[医药卫生—肿瘤]