屎肠球菌自溶的基因表达差异分析  

Differential analysis of gene expression in autolysis of Enterococcus faecium

作　　者：聂俊辉 郭浩 王通 郭建军[1] 曾静[1] 袁林[1] NIE Junhui;GUO Hao;WANG Tong;GUO Jianjun;ZENG Jing;YUAN Lin(Institute of Microbiology,Jiangxi Academy of Sciences,Nanchang,Jiangxi Province 330096,China;Agriculture,Forestry and Water Science Research Center of Shangrao,Shangrao,Jiangxi Province 334000,China;Jiangxi Xinwei Biotechnology Co.,Ltd,Nanchang,Jiangxi Province 330096,China)

机构地区：[1]江西省科学院微生物研究所,江西南昌330096 [2]上饶市农林水科学研究中心,江西上饶334000 [3]江西鑫维生物技术有限公司,江西南昌330096

出　　处：《中国饲料》2022年第23期32-39,共8页China Feed

基　　金：江西省科学院科研开发专项基金博士项目(2020-YYB-08);江西省科学院重点科研开发项目(2020-YZD21);江西省科学院省级科技计划项目包干制试点示范项目重大产业技术攻关项目(2021YSBG10005);国家自然科学基金(32060772);江西省重点研发计划(20202BBF63018,20212BBF63022)。

摘　　要：为探讨屎肠球菌自溶状态下基因表达变化,利用高通量测序技术进行RNA-Seq分析。差异基因分析显示,自溶状态下共有783个基因表达出现差异,其中在自溶状态下上调和下调的基因分别为689和94个。GO功能聚类分析显示,差异基因主要富集在细胞、细胞组分、生物合成、有机物质生物合成几个方面。KEGG富集分析发现,差异基因主要参与了核糖体、氨基酰基-tRNA生物合成、嘌呤代谢、肽聚糖生物合成、嘧啶代谢、同源重组、氨基酸合成等通路,且下调的差异基因主要富集在碳代谢、糖酵解/糖异生、丙酮酸代谢等能量代谢相关通路。说明屎肠球菌自溶状态下代谢出现了明显改变,对碳源的利用不足和合成能力的下降可能是导致屎肠球菌自溶发生的重要原因。通过分析屎肠球菌自溶状态下基因的表达情况,得到了充分的基因数据,为进一步研究屎肠球菌的自溶发生机制提供了基础。To better understand the gene expression changes of Enterococcus faecium in autolysis,highthroughput sequencing were adopted to investigate differentially expressed genes by using RNA sequencing approach. GO functional clustering analysis showed that the differential genes were mainly enriched in cell,cell part,biosynthetic process,and organic substance biosynthetic process. KEGG enrichment analysis found that the differential genes were mainly involved in ribosome,aminoacyl-tRNA biosynthesis,purine metabolism,peptidoglycan biosynthesis,pyrimidine metabolism,homologous recombination,and biosynthesis of amino acids,and the downregulated differential genes were mainly enriched in energy metabolism related pathways such as carbon metabolism,glycolysis/gluconeogenesis,and pyruvate metabolism. This suggested that the metabolism of Enterococcus faecium under autolysis has changed significantly,and the underutilization of carbon sources and the decrease of synthenicity capacity may be important reasons for the autolysis of Enterococcus faecium. In this study,a lot of genetic data were obtained by analyzing transcriptional expressions in the autolysis state of Enterococcus faecium,which may provide important basis in further revealing the autolysis mechanisms of Enterococcus faecium.

关 键 词：屎肠球菌 自溶 转录组 差异表达基因 GO功能 

分 类 号：Q78[生物学—分子生物学]