外源性海藻糖强化好氧脱氮菌处理高盐废水的菌群特性  

Characterization of trehalose enhanced aerobic denitrifying bacteria for highsalt wastewater treatment

作　　者：郭雷 肖芃颖[1] 袁港 季虹谷 向施吉 苏莉洪 GUO Lei;XIAO Pengying;YUAN Gang;JI Honggu;XIANG Shiji;SU Lihong(School of Chemistry and Chemical Engineering,Chongqing University of Technology,Chongqing 400054,China)

机构地区：[1]重庆理工大学化学化工学院,重庆400054

出　　处：《环境工程学报》2022年第10期3448-3459,共12页Chinese Journal of Environmental Engineering

基　　金：重庆市自然科学基金资助项目(cstc2020jcyj-msxmX0566);重庆市教委科学技术研究计划项目(KJQN202001138);重庆理工大学研究生创新项目资助(clgycx20203091,clgycx20203073)。

摘　　要：为提升高盐胁迫下好氧脱氮功能菌群的富集丰度及脱氮性能,解决高盐废水生物脱氮效率低的问题,助推好氧脱氮功能菌在高盐废水处理的工程应用,将海藻糖添加到已富集好氧脱氮功能菌的膜曝气生物膜反应器(membrane aerobic biofilm reactor,MABR)中,构建高盐废水的海藻糖生物强化处理系统,从反应器脱氮性能、菌群多样性以及脱氮功能基因丰度等方面探究高盐废水中海藻糖对好氧脱氮菌的强化机制。结果表明:实验组(C_(40)、C_(120)、C_(360)和C_(1080))中NH_(4)^(+)-N、TN和COD去除率相较对照组(C_(0))分别提高了10.70%、32.72%、27.36%、19.45%,8.32%、28.36%、22.53%、17.63%和12.09%、31.14%、25.27%、25.06%;外加海藻糖提高了菌群在高盐废水中的脱氮效率,浓度为120μmol·L^(-1)时,NH_(4)^(+)-N去除率最高可提升32.72%。高通量测序分析显示:在高盐环境胁迫下,海藻糖浓度对群落结构及丰度存在显著影响,C_(40)和C_(120)提高了异养硝化-好氧反硝化(heterotrophic nitrification-aerobic denitrification,HN-AD)菌(Pseudofulvimonas、Rhodobacteraceae、Paracoccus、Parapusillimonas和Flavobacterium)的相对丰度,而C_(360)和C_(1080)有利于异养反硝化菌(heterotrophic denitrified bacteria,HDB)(Enterococcu、Nitrincola、Truepera、Fusibacter)的富集;海藻糖浓度显著影响高盐废水中脱氮菌群的组成与丰度。PICRUSt1结果显示:添加海藻糖有效提高以HN-AD菌和HDB为主的脱氮菌群的反硝化活性,在C_(40)和C_(120)中,与HNAD菌关联的硝化基因(hao)和反硝化基因(nasA、napA和napB)相对丰度提高,此时HN-AD途径得到增强;而与HDB关联的反硝化基因(narG、narH和narI)相对丰度在C_(360)和C_(1080)增加,进一步说明加入高浓度海藻糖更有利于加强异养反硝化途径;海藻糖浓度为120μmol·L^(-1)时,脱氮基因相对丰度最高,最大程度地加快了好氧脱氮菌群的硝化和反硝化进程。本研究结果可为好氧脱氮菌在�In order to enhance the enrichment abundance and denitrification performance of aerobic denitrifying functional bacteria under high-salt stress,which can solve the problem of low efficiencies of biological denitrification of high-salt wastewater and promote the engineering application of aerobic denitrifying functional bacteria in high-salt wastewater treatment,the trehalose was added to the membrane aerobic biofilm reactor(MABR)with enriching aerobic denitrifying functional bacteria.The bioaugmentation mechanism of trehalose on aerobic denitrifying bacteria in high-salt wastewater was investigated in terms of nitrogen removal performance,diversity of bacterial population and abundance of nitrogen removal functional genes.The results showed that the removal rates of NH_(4)^(+)-N,TN and COD in the experimental groups(C_(40),C_(120),C_(360) and C_(1080))increased by 10.70%,32.72%,27.36%,19.45%and 8.32%,28.36%,22.53%,17.63%and 12.09%,31.14%,25.27%、25.06%,compared to the control group(C_(0)),respectively.The addition of trehalose improved the denitrification efficiency of the bacterial community in high-salt wastewater,and the NH_(4)^(+)-N removal rate increased by up to 32.72%at a concentration of 120μmol·L^(-1).High-throughput sequencing analysis revealed that under the high salinity stress,different added trehalose concentrations significantly affected bacterial community structure and abundances.Groups C_(40) and C_(120) increased the relative abundances of heterotrophic nitrification-aerobic denitrification(HN-AD)bacteria(including Pseudofulvimonas,Rhodobacteraceae,Paracoccus,Parapusillimonas,and Flavobacterium),while groups C_(360) and C_(1080) favored heterotrophic denitrified bacteria(HDB)(including Enterococcus,Nitrincola,Truepera,and Fusibacter).This demonstrated that trehalose concentration significantly affected the composition and abundance of nitrification and denitrification bacteria in high-salt wastewater.PICRUSt1 suggested that the addition of trehalose effectively increased the denitrification

关 键 词：海藻糖 异养硝化-好氧反硝化 高盐废水 微生物多样性 功能基因 

分 类 号：X703.1[环境科学与工程—环境工程]