阿克苏地区幽门螺杆菌CagA、VacA基因分型与胃溃疡及胃癌的关系  

Relationship between Helicobacter pylori CagA and VacA genotyping with gastic ulcer and gastric cancer in Aksu area

作　　者：郭新文[1] 马文颖 王隽[1] 古丽米热·艾海提 朱国林 陈长凯 GUO Xinwen;MA Wenying;WANG Jun;Gulimire Aihaiti;ZHU Guolin;CHEN Changkai(Department of Gastroenterology,Aksu Prefecture First People′s Hospital,Aksu,Xinjiang 842008,China)

机构地区：[1]新疆维吾尔自治区阿克苏地区第一人民医院消化内科,新疆阿克苏842008

出　　处：《国际检验医学杂志》2022年第24期3022-3027,共6页International Journal of Laboratory Medicine

基　　金：2019年度阿克苏地区人才项目扶持基金(2021-第13号)。

摘　　要：目的探讨阿克苏地区幽门螺杆菌细胞毒素相关基因A(CagA)、空泡细胞毒素A(VacA)基因分型与胃溃疡及胃癌的关系。方法选取2020年6月至2021年8月在该院接受胃镜检查的276例患者为研究对象,其中90例胃溃疡(胃溃疡组)、86例胃癌(胃癌组)、100例胃组织正常(正常对照组),胃溃疡组与胃癌组幽门螺杆菌检测阳性。设计引物进行各组胃组织幽门螺杆菌CagA、VacA PCR扩增及基因型判定,通过计算比值比(OR)和95%置信区间(CI)评估幽门螺杆菌CagA、VacA多态性与胃溃疡、胃癌的关系,采用多因素Logistic回归分析探讨CagA、VacA多态性位点的基因型与胃癌的关系,采用χ^(2)检验分析幽门螺杆菌CagA、VacA多态性与胃癌患者临床病理特征的关系。提取胃癌组织进行原代培养,PCR检测VacA处理后自噬相关基因SIRT1、FOXO1、P62 mRNA的表达情况,探讨VacA是否经由SIRT1、FOXO1通路促进胃癌细胞的自噬。结果胃癌组CagA AA基因型频率高于胃溃疡组、正常对照组,GG基因型频率低于胃溃疡组和正常对照组,差异均有统计学意义(P<0.05);胃溃疡组CagA AA基因型频率高于正常对照组,GG基因型频率低于正常对照组,差异均有统计学意义(P<0.05);CagA AA基因型携带者胃溃疡(OR=10.40,95%CI:5.89~14.70,P<0.05)、胃癌(OR=10.60,95%CI:4.69~21.26,P<0.05)的发病风险增加。胃癌组VacA s1m1基因型频率高于胃溃疡组与正常对照组(P<0.05),VacA s2m2基因型频率低于胃溃疡组与正常对照组(P<0.05);胃溃疡组VacA s1m1基因型频率高于正常对照组,VacA s2m2基因型频率低于正常对照组,差异有统计学意义(P<0.05);VacA s1m1基因型携带者胃溃疡(OR=11.27,95%CI:5.25~13.22,P<0.05)、胃癌(OR=9.26,95%CI:4.19~15.66,P<0.05)的发病风险增加。多因素Logistic回归分析显示:CagA AA基因型、VacA s1m1基因型是胃癌发生的危险因素(P<0.05)。CagA、VacA基因分型在不同性别、年龄胃癌患者中差异均无统计学意义(PObjective To investigate the relationship between Helicobacter pylori cytotoxin associated gene A(CagA)and vacuolar cytotoxin A(VacA)genotyping with gastic ulcer and gastric cancer in Aksu area.Methods A total of 276 patients with gastroscopic examination in this hospital from June 2020 to August 2021 were selected as the research subjects,including 90 cases of gastric ulcer(gastric ulcer group),86 cases of gastric cancer(stomach cancer group)and 100 cases of normality(normal control group).The ulcer group and gastric cancer group were positive for Helicobacter pylori.The primers were designed for PCR amplification and genotype determination of Helicobacter pylori CagA and VacA in gastric tissues of each group.The odds ratio(OR)and 95%confidence interval(CI)were calculated to evaluate the relationship between Helicobacter pylori CagA,VacA polymorphism with gastic ulcer and gastric cancer.The multivariate logistic regression analysis was used to explore the relationship between the genotype of CagA and VacA polymorphism loci with gastric cancer,and theχ^(2) test was used to analyze the relationship between Helicobacter pylori CagA and VacA polymorphism with clinicopathological feature in the patients with gastric cancer.The gastric cancer tissues were extracted for conducting the primary culture,and the expression levels of autophagy related genes SIRT1,FOXO1 and P62 mRNA after VacA treatment were detected by PCR to explore whether VacA promoting autophagy of gastric cancer cells through SIRT1 and FOXO1 pathways.Results The frequency of CagA AA genotype in the gastric cancer group was higher than that in the gastic ulcer group and normal control group,and the frequency of GG genotype was lower than that in the gastic ulcer group and normal control group,and the differences were statistically significant(P<0.05).The frequency of CagA AA genotype in the gastric ulcer group was higher than that of the normal control group,and the frequency of the GG genotype was lower than that of the normal control group,and the di

关 键 词：幽门螺杆菌 细胞毒素相关基因A 空泡细胞毒素A 胃溃疡 胃癌 阿克苏地区 

分 类 号：R573.1[医药卫生—消化系统] R735.2[医药卫生—内科学]