基于超高效液相色谱-质谱技术分析蜱传脑炎患者脂质代谢分布  

Lipid metabolism distribution in patients with tick-borne encephalitis based on liquid chromatography-mass spectrometry

作　　者：陆德生 牛艺卿 张仕华 王晓艳 孙辉 郑海军 李翔 肖翔 杜彦丹 Lu Desheng;Niu Yiqing;Zhang Shihua;Wang Xiaoyan;Sun Hui;Zheng Haijun;Li Xiang;Xiao Xiang;Du Yandan(Inner Mongolia Forestry General Hospital,Hulunbuir 022150,China)

机构地区：[1]内蒙古林业总医院,呼伦贝尔022150

出　　处：《中华实验和临床病毒学杂志》2022年第5期541-546,共6页Chinese Journal of Experimental and Clinical Virology

基　　金：内蒙古自然科学基金(2019MS08174)。

摘　　要：目的分析蜱传脑炎患者的代谢情况,研究蜱传脑炎对脂质代谢的影响,并阐明可能的发病机制。方法基于液相色谱-质谱(liquid chromatography-mass spectrometry,LC-MS)联用技术对呼伦贝尔地区的50名蜱传脑炎患者及39名健康组样本进行脂质组学研究。利用主成分分析和正交偏最小二乘-判别分析等多元统计分析方法,对患者的血清样本进行分析。结果在样本中共检测出465个质谱峰,鉴定出磷脂及甘油酯等代谢物。对显著变化(P<0.01,VIP>1,FC>2)的差异物进行鉴定,共筛选出26种差异代谢物,与机体的抗炎等代谢通路存在一定相关性,其中磷脂酰胆碱、磷脂酰乙醇胺等磷脂类整体趋于上调[log2(Fold change)>0],甘油二酯、甘油三酯等甘油酯类呈现下降趋势[log2(Fold change)<0]。且所得潜在标志物的AUC值为0.999,可用于疾病组与健康组的诊断。结论经病毒侵染后,人体内磷脂代谢及甘油酯代谢通路会发生一定变化,且所得差异代谢物可作为潜在标志物可用于蜱传脑炎疾病诊断,为疾病进一步研究提供理论依据。Objective To analyze the metabolic status of forest encephalitis patients,research the effect of forest encephalitis on lipid metabolism,and clarify the possible pathogenesis.Methods Based on liquid chromatography-mass spectrometry(LC-MS),lipidomics analysis was performed in 50 patients with tick-borne encephalitis and 39 healthy samples from Hulunbuir region.The patients′serum samples were analyzed by utilizing the multivariate statistical analysis method such as principal component analysis(PCA)and orthogonal partial least squares-discriminant analysis(OPLS-DA).Results A total of 465 peaks were detected in the samples,and metabolites such as phospholipids and glycerides were identified.To identify the significant differential metabolites(P<0.01,VIP>1,FC>2),a total of 26 biomarkers were screened,which phospholipids tend to be upregulated[log2(Fold change)>0],such as phosphatidylcholine(PC)and phosphatidylethanolamine(PE);while glycerides tend to be decreased[log2(Fold change)<0],such as diacylglycerol(DAG)and triacylglycerol(TAG),and those metabolites were closely related to anti-inflammatory and other metabolic pathways.The AUC value of potential biomarkers obtained was 0.999,which could be used for diagnosis of disease group and healthy group.Conclusions The study showed that phospholipid metabolism and glyceride metabolism pathways would have some change after human infected by virus,and the differential metabolites could be used as potential markers for the diagnosis of tick-borne encephalitis,which provide a theoretical basis for disease research.

关 键 词：蜱传脑炎 液相色谱-质谱 脂质组学 

分 类 号：R742.9[医药卫生—神经病学与精神病学]