Application of Flow Cytometry in Examination of Immunophenotypes in Human Cells  

作　　者：Zebo HUANG Aoxiang FENG Yuhan SHI Haoxian WANG Guanghong WU 

机构地区：[1]College of Life Science,South China Agricultural University,Guangzhou 510642,China

出　　处：《Agricultural Biotechnology》2022年第6期145-148,共4页农业生物技术（英文版）

基　　金：Supported by The 2021 Undergraduate Innovation and Entrepreneurship Training Program of Guangdong Province(S202110564094);Horizontal Project of South China Agricultural University(h20220138).

摘　　要：[Objectives]This study was conducted to establish a method for detecting the immunophenotypes of venous blood CIK cells in healthy donors and patients with triple-negative breast cancer or lung cancer by flow cytometry,and to further analyze and discuss the proportion of cellular immunophenotypes such as CD3^(+),CD4^(+),CD8^(+),CD3^(+)CD8^(+),CD3^(+)CD56^(+)in CIK cells.[Methods]Human venous blood was drawn,then anticoagulated with heparin and isolated with lymphocyte isolation solution,and the relevant immunophenotypes were detected by flow cytometry after 21 d of culture.[Results]The expression of CD3^(+)CD8^(+)and CD3^(+)CD56^(+)in the venous blood CIK cells was significantly higher in healthy donors than that in triple-negative breast and lung cancer patients.[Conclusions]CD3^(+)CD8^(+)and CD3^(+)CD56^(+)CIK cells have high anti-tumor activity.

关 键 词：Flow cytometry IMMUNOPHENOTYPE Cluster of differentiation CIK 

分 类 号：R730.3[医药卫生—肿瘤]