糖皮质激素对阿霉素肾病大鼠龈沟液中IL-6及TNF-α的影响  

Effects of Glucocorticoids on IL-6 and TNF-αof Gingival Crevicular Fluid in Rat Model of Adriamycin-Induced Nephropathy

作　　者：郭乐乐 侯月洁 徐文靖[1] GUO Lele;HOU Yuejie;XU Wenjing(Tianjin Children’s Hospital,Tianjin City 300000)

机构地区：[1]天津市儿童医院,300000

出　　处：《医学理论与实践》2022年第24期4144-4147,共4页The Journal of Medical Theory and Practice

摘　　要：目的:探讨应用糖皮质激素治疗肾病综合征大鼠对龈沟液中白细胞介素-6(IL-6)及肿瘤坏死因子α(TNF-α)含量的影响,以了解牙周炎与肾病及糖皮质激素应用之间的相关性。方法:60只WISTAR大鼠随机分为两组:正常组、肾病组,每组30只。肾病组采用间隔1周2次尾静脉注射盐酸阿霉素4mg/kg建立肾病综合征模型,两组各随机抽取10只处死,制作肾脏病理切片。正常组20只大鼠随机分为空白对照组、糖皮质激素组,每组10只;肾病组分为阿霉素肾病组、阿霉素肾病+激素灌胃组,每组10只。糖皮质激素组及阿霉素肾病+激素灌胃组建模后给予甲泼尼龙30mg/(kg·d)灌胃,空白对照组及阿霉素肾病组等体积生理盐水灌胃,连续10周,1次/d。采取大鼠龈沟液,ELISA法检测其中IL-6、TNF-α水平;切取上颌骨牙周组织,制作切片进行组织学观察。结果:糖皮质激素组、阿霉素肾病组、阿霉素肾病+激素灌胃组龈沟液中IL-6、TNF-α含量均高于空白对照组(P<0.05),其中阿霉素肾病+激素灌胃组的IL-6、TNF-α量明显高于其余三组(P<0.01),但糖皮质激素组与阿霉素肾病组的IL-6、TNF-α含量无统计学差异(P>0.05)。组织学结果:阿霉素肾病+激素灌胃组相对于其他组别牙周炎反应最为严重。结论:阿霉素肾病大鼠存在牙周异常,大剂量应用糖皮质激素治疗后牙周异常加重,提示肾病综合征大鼠牙周组织改变受肾病综合征疾病本身和激素治疗的双重影响。Objective:The study is aimed to evaluate the effect of IL-6 and TNF-αin gingival crevicular fluid of periodontitis in rats with acute Adriamycin-induced nephrotoxicity(ADR)treated by glucocorticoids,to realize the correlation between periodontitis nephrotic and glucocorticoid applications.Methods:60 WISTAR rats were randomly divided into two groups:normal group,nephropathy group,30 rats in each group.The rats in the nephropathy group were given 4mg/kg adriamycin injection via tail vein to establish ADR model at the interval of 2 weeks.After establishment of ADR model,the rats of the two groups were randomly select 10 rats to sacrificed and make the kidney pathological sections.Remaining 40 rats,20 rats in the normal group were randomly divided into control group,glucocorticoid group,10 rats in each group.20 rats in the nephropathy group were divided into ADR group,ADR+glucocorticoids-treated group,10 rats in each group.Glucocorticoid group and ADR+glucocorticoids-treated group were given methylprednisolone 30mg/(kg·d)for 10 weeks,control group and ADR group were given equal volume of normal saline.After 10 weeks,values of IL-6 and TNF-αwere analyzed by ELISA,the alveolar bone in four groups were collected to make histological slices.Results:IL-6 and TNF-αwere higher in glucocorticoids group,ADR group and ADR+glucocorticoids-treated group than control group(P<0.05).IL-6 and TNF-αwere significantly higher in ADR+glucocorticoids-treated group than other three groups(P<0.01).But there was no significant difference between glucocorticoids group and ADR group(P>0.05).Histomorphometric:compared with other three groups,periodontal inflammation is more serious in ADR+glucocorticoids-treated group.Conclusion:There is periodontitis in rat model of ADR.High-dose hormone therapy may promote the occurrence of periodontitis.The expression of IL-6 and TNF-αmay be associated with nephrotic syndrome and the use of glucocorticoids.

关 键 词：糖皮质激素 阿霉素肾病 IL-6 TNF-Α 龈沟液 

分 类 号：R-33[医药卫生]