基于CANON系统颗粒污泥耦合生物膜工艺脱氮性能及微生物特征研究  

Research on nitrogen removal performance and microbial characteristics of granular sludge coupled biofilm process based on CANON system

作　　者：韩松 李凯 王志康 任治军 李雪莹 HAN Song;LI Kai;WANG Zhikang;REN Zhijun;LI Xueying(School of Ecological and Environmental Engineering,Guizhou Minzu University,Guiyang 550025,China;Key Laboratory of State Ethnic Affairs Commission􀏑Karst Environmental Geological Disaster Prevention Laboratory,Guiyang 550025,China)

机构地区：[1]贵州民族大学生态环境工程学院,贵阳550025 [2]国家民委重点实验室:喀斯特环境地质灾害防治实验室,贵阳550025

出　　处：《东北农业大学学报》2022年第11期77-85,共9页Journal of Northeast Agricultural University

基　　金：国家自然科学基金项目(42107104);贵州省科技计划项目(黔科合基础[2019]1153号)。

摘　　要：为实现自养生物脱氮,通过颗粒污泥与生物膜进行同步驯化构建一种GSBR反应器。研究进水NH_(4)^(+)-N浓度、DO浓度、上升流速对GSBR反应器脱氮性能的影响,并采用SEM、高通量测序等技术分析污泥表观特征及微生物组成。结果表明,进水NH_(4)^(+)-N浓度、DO浓度、上升流速对GSBR反应器脱氮性能具有重要影响,分别在150 mg·L^(-1)、0.8 mg·L^(-1)、3.5 m·h^(-1)时脱氮性能较好。系统总氮去除负荷最大值为1.76 kgN(m3·d)^(-1),高于生物膜系统并略低于颗粒污泥系统,抗冲击负荷性能及对DO适应范围相对颗粒污泥更优,低水平时DO浓度及上升流速提升促进亚硝化并抑制Anammox。污泥形态研究表明,GSBR反应器可实现颗粒污泥与生物膜同步驯化,接种后颗粒污泥平均粒径增加50%,颗粒污泥与生物膜表观形态差异显著,高通量测序结果显示AOB主要分布在生物膜中,AnAOB则在生物膜与颗粒污泥中均有分布,尤其以颗粒污泥分布最高,生物膜与颗粒污泥协同完成系统自养脱氮过程。研究为CANON工艺工程应用研究提供参考。In order to achieve nitrogen removal by autotrophs,a GSBR reactor was constructed by simultaneous acclimation of granular sludge and biofilm.The effects of the influent NH_(4)^(+) -N concentration,DO concentration,and rising flow rate on the nitrogen removal were studied respectively,and the appearance characteristics,microbial composition of the sludge were analyzed by SEM and high-throughput sequencing.The results showed that the influent NH_(4)^(+) -N concentration,DO concentration,and rising flow rate had important roles on the nitrogen removal performance of the GSBR,which were respectively 150 mg·L^(-1),0.8 mg·L^(-1),3.5 m·h^(-1),when the nitrogen removal performance was better.The maximum TN removal load was 1.76 kgN(m3·d)^(-1),which was higher than that of the biofilm and slightly lower than that of the granular sludge,but the impact load resistance and DO adaptation were better than those of granular sludge.The increase of DO concentration and rising flow rate at low level promoted nitrosation and inhibited Anammox.The study of sludge morphology showed that GSBR reactor could realize the simultaneous domestication of granular sludge and biofilm,the average particle size of granular sludge increased by 50%after inoculation,and the appearance of granular sludge and biofilm showed a significant difference.The high-throughput results showed that AOB was mainly distributed in biofilms,but AnAOB was distributed in both biofilm and granular sludge,especially in granular sludge,and biofilm and granular sludge cooperated to complete the autotrophic nitrogen removal process.This study will provide a reference for the research of CANON process engineering application.

关 键 词：GSBR CANON 颗粒污泥 微生物多样性 

分 类 号：X52[环境科学与工程—环境工程]