Trace antibiotics perturb the metabolism of Escherichia coli  

痕量抗生素扰动大肠杆菌代谢

作　　者：Dongyang Ye Chengfei Wang Xiaowei Li Liang Zhao Saiwa Liu Jingjing Du Xixi Jia Zhinan Wang Lu Tian Jian Xu Jing Li Zuhao Yan Jiangyi Ding Jianzhong Shen Xi Xia 冶冬阳;王成飞;李晓薇;赵亮;刘赛娃;杜静静;贾希希;王智楠;田璐;许健;李静;严祖浩;丁江伊;沈建忠;夏曦(College of Veterinary Medicine,China Agricultural University,Beijing 100193,China)

机构地区：[1]College of Veterinary Medicine,China Agricultural University,Beijing 100193,China

出　　处：《Science Bulletin》2022年第21期2158-2161,共4页科学通报（英文版）

基　　金：supported by the National Natural Science Foundation of China(32072916)。

摘　　要：Since their initial clinical implementation,antibiotics have become one of the foundations of modern medicine.Antibiotics used in humans and animals are excreted through urine and feces[1],which enter the surface water or groundwater,where they eventually contaminate the water supply system.Trace levels of antibiotics,usually at nanogram per liter,have been reported in both tap and bottled water[2,3]and these compounds are often difficult to be completely removed,even after the advanced purification provided by high-end laboratory systems[4].Thus experimental reagents and materials might be contaminated when produced using water with trace antibiotic contamination.水中痕量抗生素污染对科研实验的影响尚未引起重视.本研究发现,大多数抗生素在细菌培养基中经过高压灭菌处理后没有完全降解;使用含有ng/L浓度抗生素的水配制细菌培养基,可以引起大肠杆菌代谢组和转录组的复杂变化,并且这些代谢扰动与亚治疗浓度(μg/L)抗生素暴露导致的代谢扰动并不完全一致;进一步研究证明,痕量抗生素引起的代谢变化能够干扰耐药大肠杆菌代谢组学分析的结果.以上结果表明,开展细菌代谢及耐药性机制研究时,需要关注水中痕量抗生素污染的影响.

关 键 词：细菌培养基 科研实验 细菌代谢 治疗浓度 高压灭菌 抗生素污染 代谢变化 代谢组 

分 类 号：X172[环境科学与工程—环境科学]