‘阳光玫瑰’葡萄一年两收果实类黄酮组分及含量 差异分析  

Difference in Flavonoid Composition and Content Between Summer and Winter Grape Berries of Shine Muscat Under Two-Crop-a-Year Cultivation

作　　者：王博[1] 覃富强 邓凤莹 罗惠格 陈祥飞 成果[2] 白扬 黄小云 韩佳宇[2] 曹雄军[2] 白先进[4] WANG Bo;QIN FuQiang;DENG FengYing;LUO HuiGe;CHEN XiangFei;CHENG Guo;BAI Yang;HUANG XiaoYun;HAN JiaYu;CAO XiongJun;BAI XianJin(College of Agriculture,Guangxi University,Nanning 530004;Grape and Wine Research Institute,Guangxi Academy of Agriculture Science,Nanning 530007;Guangxi Zhencheng Agricultural Co.,Ltd.,Nanning 530007;Guangxi Academy of Agriculture Science,Nanning 530007)

机构地区：[1]广西大学农学院,南宁530004 [2]广西农业科学院葡萄与葡萄酒研究所,南宁530007 [3]广西真诚农业有限公司,南宁530007 [4]广西农业科学院,南宁530007

出　　处：《中国农业科学》2022年第22期4473-4486,共14页Scientia Agricultura Sinica

基　　金：国家自然科学基金(31960572);广西重点研发计划(桂科AB21196042);广西农业科学院基本科研业务专项资助项目(2021YT126)。

摘　　要：【目的】以3年生一年两收栽培‘阳光玫瑰’葡萄为试材,探究夏果与冬果基本理化指标、类黄酮物质组分及含量的差异,为‘阳光玫瑰’葡萄一年两收栽培的品质调控提供理论依据。【方法】记录‘阳光玫瑰’葡萄全生育期日照时数、光照度、温度、降雨量等气候数据,在夏果与冬果的幼果期、膨大期、软化期、开始成熟期、成熟期测定果实基本理化指标,并利用高效液相色谱质谱(LC-MS/MS)联用技术检测各时期果皮中黄酮醇和黄烷醇的组分及含量。【结果】气候因子方面,‘阳光玫瑰’葡萄夏果生长前期光照度弱、温度低,后期光照度强、温度高,而冬果与之相反;夏果生长期平均日照时数、平均温度、有效积温大于冬果,但降雨量低于冬果,开始成熟期至成熟期的夏果水热系数高于冬果。基本品质方面,成熟期夏果可溶性固形物含量显著高于冬果,果皮厚度显著低于冬果,果实单粒重、果实横纵径、可滴定酸含量在夏果与冬果中无显著差异。黄酮醇的组分及含量方面,夏果与冬果的总黄酮醇含量整体呈下降趋势,夏果各时期总黄酮醇含量均显著高于冬果,夏果中黄酮醇主要成分为槲皮素-3-O-葡萄糖苷,冬果中黄酮醇以山奈酚-3-O-半乳糖苷为主。黄烷醇的组分及含量方面,夏果与冬果的总黄烷醇含量也均呈下降趋势,夏果和冬果果皮中均检测到8种相同的黄烷醇物质,主要成分为儿茶素、表儿茶素和原花青素B 1。夏果果实发育各时期果皮中总黄烷醇含量以及儿茶素、表儿茶素、原花青素B 1含量均显著低于冬果,果实成熟期夏果没食子儿茶素、表没食子儿茶素、表儿茶素没食子酸酯、表没食子儿茶素没食子酸酯、原花青素B 2含量显著高于冬果。主成分分析表明夏果与冬果的黄酮醇类物质组分有一定差异。回归分析表明,儿茶素、槲皮素-3-O-葡萄糖苷、原花青素B 1�【Objective】3-year-old Shine Muscat grape under two-crop-a-year cultivation was used as the material to investigate the differences of physical and chemical indexes of basic quality,flavonoid components and contents between summer grape and winter grape,which would provide the theoretical basis for the quality control of Shine Muscat grape under two-crop-a-year cultivation.【Method】The climatic data,such as the sunshine duration,light intensity,temperature and rainfall during the whole growth period of Shine Muscat grape,were recorded.The physical and chemical indexes of basic quality in berries of summer and winter grapes of Shine Muscat were determined at the young fruit stage,expansion stage,softening stage,beginning maturity stage,and maturity stage,respectively.Meanwhile,the components and contents of flavonols and flavanols in the peel of summer and winter grapes of Shine Muscat grape were detected by LC-MS/MS.【Result】In terms of the climate factors,the summer grape of Shine Muscat displayed weak illumination and low temperature at early growth stage but strong illumination and high temperature at late growth stage of the whole developing period,while the winter grape was opposite.The average sunshine duration,average temperature and effective accumulated temperature in the growth period of summer grape were higher than those of winter grape,but the rainfall was lower than that of winter grape.The hydrothermal coefficient of summer grape was higher than that of winter grape from the beginning of maturity stage to maturity stage.In terms of the basic quality,the content of soluble solid of summer grape was significantly higher than that of winter grape at maturity stage,and the peel thickness of summer grape was significantly lower than that of winter grape.There was no significant difference in the single berry weight,fruit equatorial and longitudinal diameter and titratable acid content between summer and winter grape.In terms of the components and contents of flavonols,the content of total flavo

关 键 词：‘阳光玫瑰’葡萄 一年两收 气候 果皮 黄酮醇 黄烷醇 

分 类 号：S663.1[农业科学—果树学]