“川芎-当归”药对主要活性成分的网络药理学研究  

Study on the network pharmacology of the main active components of"ligusticum-angelica"herb couple

作　　者：张莹莹 张科[2] 宋崟 马红英 邵佳[1] 张弋[1] ZHANG Yingying;ZHANG Ke;SONG Yin;MA Hongying;SHAO Jia;ZHANG Yi(Department of Pharmacy,Tianjin First Cenral Hospital,Tianjin 300192,China;Department of Pharmacy,Tianjin Medical University General Hospital,Tianjin 300052,China)

机构地区：[1]天津市第一中心医院药学部,天津300192 [2]天津医科大学总医院药剂科,天津300052

出　　处：《安徽医药》2023年第1期19-24,共6页Anhui Medical and Pharmaceutical Journal

摘　　要：目的应用网络药理学方法探究川芎-当归药对的主要活性成分、靶点和药理作用机制。方法研究时间为2020年9—12月。首先以“川芎”“当归”为关键词,在TCMSP 2.3数据库中检索药材的成分、靶点和对应疾病数据,构建“药物-成分靶点-疾病”网络,进行基因本体(gene ontology,GO)分类富集分析、京都基因和基因组百科全书(kyoto encyclopedia of genes and genomes,KEGG)通路富集分析,探究“川芎-当归”药对作用机制。结果“药物-成分-靶点-疾病”网络包含2个药物、10个活性成分,71个作用靶点,191种疾病。关键靶点涉及环氧化酶2(PTGS2)、过氧化物酶体增生激活受体γ(PPARG)、雌激素受体(ESR1)、β2肾上腺素能受体(ADRB2)、周期蛋白依赖激酶2(CDK2)、热休克蛋白90(HSP90)、促分裂原活化蛋白激酶14(MAPK14)、胆碱能受体2(CHRM2)、5羟色胺受体2A(HTR2A)、凝血因子Ⅱ受体(F2R)、盐皮质激素受体(NR3C2)、内皮型一氧化氮合酶(NOS3)等,关键疾病涉及疼痛、心血管疾病、乳腺癌、阿尔茨海默病、炎症、癌症、焦虑症、精神分裂症、前列腺癌、实体肿瘤、脑损伤等。GO富集分析得到237个条目,包括生物过程178个,分子功能26个,细胞组成33个。通路富集分析包含66条通路,主要涉及神经活性配体-受体相互作用、钙离子信号通道、癌症通路、5-羟色胺能突触、大肠癌、雌激素信号通路、心肌细胞的肾上腺素能信号、甲状腺激素信号通路、cAMP信号通路、血管内皮生长因子信号通路等。结论“川芎-当归”药对中多个成分作用于多个靶点和通路,对疼痛、心血管疾病、乳腺癌、阿尔茨海默病、炎症、癌症等多种疾病均一定的治疗作用。Objective To explore the main active components,targets and mechanism of action of Ligusticum-Angelica herb pair by using the method of network pharmacology.Methods This study starts and ends from September 2020 to December 2020.The compo‐nents,targets and corresponding disease datas of Ligusticum-Angelica were retrieved by Chinese medicine Systems pharmacology data‐base and analysis platform(TCMSP),and a"drug-component-target-disease"network was constructed,gene ontology(GO)and path‐way enrichment(KEGG)were analyzed to explore the mechanism of"Ligusticum-Angelica".Results The"drug-component-target-dis‐ease"network consisted 2 drugs,10 active components,71 active targets and 191 diseases.The key targets involved:PTGS2,PPARG,ESR1,ADRB2,CDK2,HSP90,MAPK14,CHRM2,HTR2A,F2R,NR3C2,NOS3,etc.,and key diseases included alzheimer's disease,pain,cancer,breast cancer,cardiovascular disease,anxiety,brain damage,inflammation,prostate cancer,and solid tumors.GO enrich‐ment analysis resulted in 237 items,including 178 items of biological process(BP),26 items of molecular function(MF)and 33 items of cell composition(CC).Pathway enrichment analysis included 66 KEGG pathways,including neuroactive ligand-receptor interaction,calcium signaling pathway,pathways in cancer,serotonergic synapse etc.Conclusion Multiple components of"Ligusticum-Angelica"herb pair act on multiple targets and pathways,and have therapeutic effects on pain,cardiovascular disease,breast cancer,alzheimer's disease,inflammation,cancer and other diseases.

关 键 词：川芎 当归 药对 网络药理学 靶点 信号通路 作用机制 环氧化酶2 细胞周期蛋白依赖激酶2 受体 盐皮质激素 京都基因和基因组百科全书(KEGG) 

分 类 号：R285[医药卫生—中药学]