转化生长因子β1在血小板促进甲状腺未分化癌侵袭迁移中的作用  

Role and Mechanism of TGFβ1 in Platelet Promoting Invasion and Migration of Anaplastic Thyroid Carcinoma Cells

作　　者：蒋玉娥[1] 钟兆铭 高艳章 王伟[2] 孙瑞梅[2] 孙传政[2] JIANG Yu’e;ZHONG Zhaoming;GAO Yanzhang;WANG Wei;SUN Ruimei;SUN Chuanzheng(Clinical Laboratory,The 3rd Affiliated Hospital of Kunming Medical University Tumor Hospital of Yunnan Province,Kunming Yunnan 650118;Dept.of Head and Neck Surgery SectionⅡ,The 3rd Affiliated Hospital of Kunming Medical University Tumor Hospital of Yunnan Province,Kunming Yunnan 650118;Dept.of Oncology,The 1st Affiliated Hospital of Kunming Medical University,Kunming Yunnan 650032,China)

机构地区：[1]昆明医科大学第三附属医院/云南省肿瘤医院检验科,昆明云南650118 [2]昆明医科大学第三附属医院/云南省肿瘤医院头颈外二科,昆明云南650118 [3]昆明医科大学第一附属医院肿瘤内科,昆明云南650032

出　　处：《昆明医科大学学报》2022年第12期1-5,共5页Journal of Kunming Medical University

基　　金：国家自然科学基金资助项目(81560470,81773127);云南省科技厅-昆明医科大学应用基础研究联合专项基金资助项目(2018FE001-058,2019FE001-075)。

摘　　要：目的探索肿瘤患者血小板(platelet,PLT)是否通过分泌转化生长因子β1(transforming growth factorβ1,TGFβ1)参与调控甲状腺未分化癌(anaplastic thyroid carcinoma,ATC)的转移。方法通过ELISA和Real-time PCR实验测定ATC细胞上清TGFβ1蛋白和mRNA表达情况;通过Transwell迁移实验、划痕实验和Western Blot方法探究TGFβ1对ATC细胞迁移及侵袭能力的影响及其作用机制。结果PLT与ATC细胞共培养上清液中TGFβ1蛋白水平显著上调(P<0.001)而mRNA水平不变;PLT或rhTGFβ1可使穿过小室的ATC细胞显著增加(P<0.001),提示PLT或rhTGFβ1能显著增强ATC细胞的迁移能力(P<0.001);划痕实验也证实rhTGFβ1可显著增强ATC细胞的迁移能力(P<0.001)。结论PLT可通过分泌TGFβ1诱导ATC细胞EMT进程最终增强ATC细胞迁移和侵袭能力,表明TGFβ1可能成为ATC患者靶向治疗的潜在靶标。Objective This study aims to explore whether platelet(PLT)is involved in regulating the progression of anaplastic thyroid carcinoma(ATC)by secreting transforming growth factorβ1(TGFβ1).Methods The levels of TGFβ1 protein and mRNA in the supernatant of ATC cells were detected by ELISA and Real-time PCR,respectively.Transwell,scratch wound test and Western Blot were used to analyze the effects of TGFβ1 on the invasive and migratory abilities of ATC cell lines and its mechanism.Results In the co-culture supernatant of PLT and ATC cells,the protein level of TGFβ1 was significantly up-regulated,but the mRNA level was unchanged.PLT or rhTGF-β1 significantly increased the number of ATC cells crossing the chamber(P<0.001),suggesting that PLT or rhTGF-β1 could significantly enhance the migration ability of ATC cells.Scratch wound assay also confirmed that rhTGF-β1 could significantly enhance the migration ability of ATC cells.Conclusion PLT can significantly promote the development of EMT in ATC cells by secreting TGFβ1,and then promote the invasion and migration of ATC cells,suggesting that TGFβ1 may be a potential target of ATC molecular targeted therapy.

关 键 词：甲状腺未分化癌 血小板 转化生长因子Β1 侵袭与迁移 

分 类 号：R736.1[医药卫生—肿瘤]