血管内皮生长因子-C对鼻咽癌CNE-2细胞小鼠移植瘤的辐射敏感性的影响  

Effect of VEGF-C on Radiation Sensitivity of Nasopharyngeal Carcinoma CNE-2 Cell Transplanted Mice

作　　者：吴锡芳[1] 汪勇[2] 彭丽莎[2] 罗洁[2] 王枫[2] WU Xifang;WANG Yong;PENG Lisha;LUO Jie;WANG Feng(Dept.of Head and Neck Surgery,The 3rd Affiliated Hospital of Kunming Medical University,Kunming Yunnan 650118;Dept.of Radiotherapy,The 1st Affiliated Hospital of Kunming Medical University,Kunming Yunnan 650032,China)

机构地区：[1]昆明医科大学第三附属医院头颈外科,云南昆明650118 [2]昆明医科大学第一附属医院放疗科,云南昆明650032

出　　处：《昆明医科大学学报》2022年第12期6-11,共6页Journal of Kunming Medical University

基　　金：云南省科技厅-昆明医科大学应用基础研究联合专项基金资助项目(2019FE001)。

摘　　要：目的探讨干扰VEGF-C表达可以增加鼻咽癌CNE-2细胞小鼠移植瘤辐射敏感性作用以及相关信号途径。方法鼻咽癌CNE-2小鼠移植瘤随机分为:瘤体组;siRNA-NC组;siRNA-VEGF-C组;照射组;siRNA-NC+照射组;siRNA-VEGF-C+照射组。观察肿瘤生长情况;采用免疫组化检测VEGF-C、ATM、P65蛋白的表达变化。结果干扰VEGF-C表达鼻咽癌CNE-2细胞小鼠移植瘤,在照射后明显肿瘤生长明显变慢。免疫组化检测结果显示:干扰VEGF-C小鼠移植瘤后,VEGF-C、ATM明显减少(P<0.05),P65增加(P<0.05);联合照射后,VEGF-C、ATM明显减少(P<0.05)。结论在鼻咽癌CNE-2细胞小鼠移植瘤中,干扰VEGF-C基因通过激活NF-kB信号通路影响下游ATM,进而增加放疗敏感性。Objective To investigate the effect of interfering with the expression of VEGF-C on the radiosensitivity of the nasopharyngeal carcinoma CNE-2 cell transplanted tumor and related signaling pathways.Methods The mice with transplanted tumors of nasopharyngeal carcinoma CNE-2 were randomly divided into tumor body group,siRNA-NC group,siRNA-VEGF-C group;irradiation group;siRNA-NC+irradiation group;siRNA-VEGF-C+irradiation group.Tumor growth was observed,and the protein expression of VEGF-C,ATM and P65 were detected by immunohistochemistry.Results Interference of VEGF-C expression in nasopharyngeal carcinoma CNE-2 cell transplanted tumors showed significantly slower tumor growth after irradiation.Immunohistochemical test results showed that after tumor transplantation of mice interfered with VEGF-C,VEGFC and ATM were significantly decreased(P<0.05),while P65 was increased(P<0.05).After combined irradiation,VEGF-C and ATM were significantly decreased(P<0.05).Conclusion In mouse nasopharyngeal carcinoma CNE-2 cell transplantation tumors,interfering with VEGF-C gene affects downstream ATM by activating NF-kB signaling pathway,thereby increasing the sensitivity of radiotherapy.

关 键 词：鼻咽癌 血管内皮生长因子-C 辐射敏感性 实验动物 

分 类 号：R739.62[医药卫生—肿瘤]