淋巴结和骨髓微环境对小鼠口腔癌细胞PCNA、P38和ERK1/2表达的影响  

Effects of lymph node and bone marrow microenvironment on the expressions of PCNA,P38 and ERK1/2 in mouse oral cancer cells

作　　者：陈冠希 王振瑞 陈鹏宁 陈昕煜 罗霖 于大海[5] Chen Guanxi;Wang Zhenrui;Chen Pengning;Chen Xinyu;Luo Lin;Yu Dahai(Department of Oral and Maxillofacial Surgery,College of Stomatology,Guangxi Medical University,Nanning 530021,China;Guangxi Key Laboratory of Oral and Maxillofacial Rehabilitation and Reconstruction,Nanning 530021,China;Guangxi Clinical Research Center for Craniofacial Deformity,Nanning 530021,China;Tai'an stomatological hospital,Tai’an 271000,China;Department of Stomatology,The First Affiliated Hospital of Guangxi Medical University,Nanning 530021,China)

机构地区：[1]广西医科大学口腔医学院/附属口腔医院口腔颌面外科,南宁530021 [2]广西口腔颌面修复与重建研究自治区级重点实验室,南宁530021 [3]广西颅颌面畸形临床医学研究中心,南宁530021 [4]泰安市口腔医院,泰安271000 [5]广西医科大学第一附属医院口腔科,南宁530021

出　　处：《广西医科大学学报》2022年第11期1706-1712,共7页Journal of Guangxi Medical University

基　　金：国家自然科学基金资助项目(No.81360407);广西自然科学基金资助项目(No.2016GXNSFDA380002)。

摘　　要：目的:探讨balb/c小鼠和balb/c裸鼠的淋巴结或骨髓微环境对小鼠口腔癌细胞增殖和休眠相关因子PCNA、P38和ERK1/2表达的影响。方法:利用transwell小室建立微环境与小鼠口腔癌细胞体外间接共培养模型,分为5组:培养基共培养组(NC组)、正常balb/c小鼠淋巴结共培养组、免疫缺陷balb/c裸鼠淋巴结共培养组、正常balb/c小鼠骨髓共培养组和免疫缺陷balb/c裸鼠骨髓共培养组。应用蛋白免疫印迹(Western blotting)于共培养后72 h检测癌细胞PCNA、P38和ERK1/2的表达。结果:标准化实验流程并成功建立不同淋巴结和骨髓微环境与小鼠口腔癌细胞体外共培养模型;Western blotting结果显示在共培养72 h后,正常小鼠淋巴结组的PCNA表达量高于骨髓组(P<0.01),而P38的表达则低于骨髓组(P<0.01),ERK1/2的表达在两组差异无统计学意义(P>0.05);裸鼠淋巴结组PCNA和P38的表达量低于骨髓组(P<0.01),而ERK1/2的表达高于骨髓组(P<0.01);正常小鼠淋巴结组PCNA和P38的表达高于裸鼠淋巴结组(P<0.05),而ERK1/2的表达差异无统计学意义(P>0.05);正常鼠骨髓组和裸鼠骨髓组PCNA、P38和ERK1/2的表达差异无统计学意义(P>0.05);ERK1/2与P38比值由高到低依次为裸鼠淋巴结组、正常鼠淋巴结组、正常鼠和裸鼠骨髓组,最低为NC组,且正常鼠骨髓组和裸鼠骨髓组的差异无统计学意义(P>0.05)。结论:不同免疫功能的微环境可以影响口腔癌细胞增殖和休眠,淋巴结微环境相比于骨髓微环境具有更强的“促增殖抑休眠”能力。Objective:To study the effects of lymph nodes or bone marrow microenvironment in balb/c mouse and balb/c nude mouse on the expressions of proliferation and dormancy-related factors of PCNA,P38 and ERK1/2 in mouse oral cancer cells.Methods:Indirect co-culture models of microenvironment and mouse oral cancer cells in vitro were established using transwell chambers and were divided into five groups:medium co-culture group(NC group),normal balb/c mouse lymph node co-culture group,immunodeficient balb/c nude mouse lymph node co-culture group,normal balb/c mouse bone marrow co-culture group and immunodeficient balb/c nude mouse bone marrow co-culture group.Western blotting was applied to detect the expressions of PCNA,P38 and ERK1/2 in oral cancer cells for 72 h after co-culture.Results:The experimental procedure was standardized and the co-culture models of different lymph nodes,bone marrow microenvironment and mouse oral cancer cells in vitro were successfully established;western blotting results showed that after co-culture for 72 h,the expression of PCNA was higher in the normal mouse lymph node group than that in the bone marrow group(P<0.01),the expression of P38 was lower than that in the bone marrow group(P<0.01),and the expression difference of ERK1/2 had no statistical significance(P>0.05);the expressions of PCNA and P38 in the lymph node group of nude mouse were lower than those in the bone marrow group(P<0.01),while the expression of ERK1/2 was higher than that in the bone marrow group(P<0.01);the expressions of PCNA and P38 in the normal mouse lymph node group were higher than those in the nude mouse lymph node group(P<0.05),while the expression difference of ERK1/2 had no statistical significance(P>0.05);the expression differences of PCNA,P38 and ERK1/2 between the normal and nude mouse bone marrow groups had no statistical significance(P>0.05);the ratios of ERK1/2 and P38 from high to low were in the nude mouse lymph node group,the normal mouse lymph node group,the normal mouse bone marrow group and the

关 键 词：口腔癌 微环境 增殖 休眠 PCNA P38 ERK1/2 

分 类 号：R739[医药卫生—肿瘤]