丁苯酞对慢性缺血性脑白质损害的保护作用研究  

Protective Effect of Butylphthalide on White Matter Damage in Chronic Cerebral Hypoperfu⁃sion

作　　者：林琅 黎红华 骆文静 吴非 LIN Lang;LI Hong-hua;LUO Wen-jing;WU Fei(Department of Neurology,General Hospital of Central Theater,PLA,Wuhan 430070,China)

机构地区：[1]中部战区总医院神经内科,武汉430011

出　　处：《神经损伤与功能重建》2022年第12期746-748,共3页Neural Injury and Functional Reconstruction

基　　金：湖北省卫生健康科研联合项目(No.WJ2019H118)。

摘　　要：目的:观察丁苯酞可否改善慢性低灌注所致脑白质损害并探讨其可能的机制。方法:72只大鼠随机分为对照组、低灌注组及丁苯酞组,每组24只。低灌注组及丁苯酞组通过阻断双侧颈总动脉血流建立慢性前循环低灌注模型,丁苯酞组于术后给予丁苯酞注射液腹腔注射。建模30 d后,通过荧光素异硫氰酸酯-葡聚糖静脉注射结合共聚焦激光扫描法检测脑微血管密度,通过伊文思蓝静脉注射后测定脑组织荧光强度检测血脑屏障完整性,通过Kluver-Barrera氏髓鞘染色检测白质纤维密度。结果:丁苯酞组大鼠脑白质微血管密度高于其他2组(均P<0.05)。低灌注组大鼠伊文思蓝浓度较对照组大鼠显著增高(P<0.01);丁苯酞组伊文思蓝浓度较对照组大鼠增高(P<0.05),但较低灌注组降低(P<0.05)。低灌注组大鼠脑白质纤维密度较对照组显著降低(P<0.01);丁苯酞组大鼠脑白质纤维密度较对照组降低(P<0.05),但较低灌注组增高(P<0.05)。结论:丁苯酞可减轻慢性低灌注状态导致的脑白质损害,其机制可能与改善微循环、减轻血脑屏障破坏有关。Objective:To estimate whether butylphthalide improves white matter damage and blood-brain barrier disruption by chronic cerebral hypoperfusion and explore its possible mechanism.Methods:72 Wistar rats were randomly split into three groups with 24 in each:sham group,hypoperfusion group,and butylphthalide group.Permanent bilateral common carotid artery occlusion was used to induce hypoperfusion of the forebrain in hypoperfusion and butylphthalide group rats.Intraperitoneal injection of butylphthalide was administered to butylphthalide group rats after surgery.Microvessel density,Kluver-Barrera’s myelin sheath staining,and quantitative measurement of pre-injected Evans Blue were performed 30 days after surgery.Results:Compared to the control and hypoperfusion group rats,the butylphthalide group rats had a higher microvessel density(P<0.05).Hypoperfusion group rats showed a higher leakage of Evans Blue dye compared to control group rats(P<0.01).Butylphthalide group rats showed a greater leakage of Evans Blue dye than control group rats(P<0.05)but a smaller leakage than hypoperfusion group rats(P<0.05).Hypoperfusion group rats had a lower white matter fiber density than control group rats(P<0.01).Butylphthalide group rats showed a lower white matter fiber density than the control group rats(P<0.05)but a higher density than hypoperfusion group rats(P<0.05).Conclusion:Butylphthalide reduced the white matter impairment caused by chronic cerebral hypoperfusion,and its key mechanism may involve the improvement of microcirculation and the reduction of damage to the blood brain barrier.

关 键 词：慢性低灌注 脑白质损害 血脑屏障 微循环 丁苯酞 

分 类 号：R741[医药卫生—神经病学与精神病学] R741.02[医药卫生—临床医学] R743