高共轭性的四苯基卟啉-Ru(Ⅱ)联吡啶配合物的合成、光学性质及光动力抗癌活性  

Highly Conjugated Tetraphenylporphyrin-Ru(Ⅱ) Bipyridine Complex:Synthesis,Optical Properties,and Photodynamic Anticancer Activity

作　　者：张劲祥 黄伟国[2] 黄嘉良[2] 麦乃歧[3] ZHANG Jing-Xiang;WONG Wai-Kwok;WONG Ka-Leung;MAK Nai-Ki(School of Chemistry and Environmental Engineering,Hanshan Normal University,Chaozhou,Guangdong 521041,China;Department of Chemistry,Faculty of Science,Hong Kong Baptist University,Hong Kong 999077,China;Department of Biology,Faculty of Science,Hong Kong Baptist University,Hong Kong 999077,China)

机构地区：[1]韩山师范学院化学与环境工程学院,潮州521041 [2]香港浸会大学理学院化学系,中国香港999077 [3]香港浸会大学理学院生物系,中国香港999077

出　　处：《无机化学学报》2022年第12期2383-2391,共9页Chinese Journal of Inorganic Chemistry

基　　金：广东省教育厅特色创新类项目(No.2018KTSCX137);广东省教育厅创新团队项目(No.2017KCXTD023);韩山师范学院博士启动项目(No.QD20180322);广东省基础与应用基础研究基金项目(No.2021A1515012358)资助。

摘　　要：利用β位吡嗪环共轭连接的卟啉-菲咯啉配体L制备了两亲性的卟啉基-钌配合物PorRu和PorZn-Ru,对其进行了核磁及质谱的表征、线性与非线性光谱分析及光动力抗癌活性研究。实验结果显示所得的2个钌配合物具有高的^(1)O_(2)量子产率(0.93、0.82)和较高的双光子吸收截面(619、621 GM),鼻咽癌HK-1细胞株的摄取量可达每10~6个细胞约22 nmol。体外光动力抗癌活性测试显示在2 J·cm^(-2)的光学剂量及4μmol·L^(-1)的给药浓度下,抑制效率分别达到(87.44±2.21)%、(45.03±2.85)%。Two amphiphilic porphyrin-ruthenium(Ⅱ) complexesPorRuandPorZn-Ruwere synthesized by the coordination of metal Ru(Ⅱ) and porphyrin-phenanthroline ligand L which is modified with conjugated pyrazine ring at the β position of porphyrin.Two compounds were characterized and evaluated by NMR and mass spectrometry,linear and nonlinear spectral analysis,and photodynamic anti-cancer activity study.Experimental results show that the two compounds had very high ^(1)O_(2) quantum yields(0.93,0.82),high two-photon absorption cross-sections(619,621 GM),and up to about 22 nmol per 10~6 cell uptakes of nasopharyngeal carcinoma HK-1 cell line.Although no obvious sub-cellular target was observed by the co-staining cell images,the high ~1Oyields and good cellular uptakes still made the ruthenium compounds have good photodynamic anticancer activity against HK-1 cells.At the light dose of 2 J·cm^(-2) and the administration concentration of 4 μmol·L^(-1),the inhibition efficiency reached(87.44±2.21)% and(45.03±2.85)% respectively.

关 键 词：卟啉 钌配合物 两亲性 双光子 光动力治疗 

分 类 号：O614.821[理学—无机化学]