外源硫化氢对盐碱胁迫下裸燕麦叶片有机酸和激素含量的影响  

Effects of Exogenous Hydrogen Sulfide on Contents of Organic Acids and Hormones in Leaves of Avena nuda under Saline-Alkali Stress

作　　者：刘建新[1] 刘瑞瑞[1] 刘秀丽[1] 欧晓彬 贾海燕[1] 卜婷[1] 李娜[1] LIU Jianxin;LIU Ruirui;LIU Xiuli;OU Xiaobin;JIA Haiyan;BU Ting;LI Na(Gansu Key Laboratory of Protection and Utilization for Biological Resources and Ecological Restoration,College of Life Sciences and Technology,Longdong University,Qingyang 745000)

机构地区：[1]甘肃省陇东生物资源保护利用与生态修复重点实验室/陇东学院生命科学与技术学院,庆阳745000

出　　处：《植物研究》2023年第1期76-89,共14页Bulletin of Botanical Research

基　　金：国家自然科学基金(31960375,31860114);甘肃省自然科学基金(20JR5RA491,20JR5RA486)。

摘　　要：为探讨外源硫化氢(H2S)对盐碱胁迫下植物有机酸和激素水平的调控效应,以裸燕麦(Avena nuda)为材料,研究喷施50μmol·L^(−1) H_(2)S供体硫氢化钠(NaHS)溶液对3.00 g·kg^(−1)盐碱胁迫下叶片有机酸、激素含量和产量性状的影响。结果表明:盐碱胁迫显著提高了琥珀酸、丁烯二酸、苹果酸、葡萄糖醛酸和总有机酸含量,显著降低了焦谷氨酸、茉莉酸-异亮氨酸(JA-Ile)、反式-玉米素(tZ)和N^(6)-(Δ^(2)-异戊烯)腺嘌呤(iP)含量。喷施NaHS溶液显著提高盐碱胁迫下裸燕麦叶片中3-羟基-3-甲基谷氨酸、吲哚乙酸(IAA)、赤霉素A_(7)(GA_(7))、茉莉酸甲酯(MJA)、iP含量和IAA/ABA比值,显著降低葡萄糖醛酸、赤霉素A_(3)(GA_(3))、赤霉素A_(4)(GA_(4))、总赤霉素(GA_(S))、1-氨基环丙烷羧酸(ACC)含量和ACC/ABA比值,而对琥珀酸、丁烯二酸、苹果酸、柠檬酸、丙二酸、泛酸、烟酸、焦谷氨酸、辛二酸、苯丙酮酸、总有机酸、赤霉素A_(1)(GA_(1))、茉莉酸(JA)、JA-Ile、脱落酸(ABA)、tZ、反式-玉米素核苷(tZR)、N^(6)-(Δ^(2)-异戊烯基)腺苷(iPA)、总茉莉酸类(JAs)、细胞分裂素(CTK)含量、GAs/ABA、JAs/ABA、CTK/ABA比值无显著影响。主成分分析表明,喷施NaHS溶液使盐碱胁迫下裸燕麦叶片中有机酸3-羟基-3-甲基谷氨酸和激素MJA、GA_(7)、tZ、IAA含量分别显著上调14.31%和41.83%、50.00%、22.97%、13.02%;而有机酸烟酸、葡萄糖醛酸和激素GA_(4)、ACC、tZR、GA_(3)含量分别显著下调16.00%、23.87%和73.53%、32.72%、50.00%、33.91%。另外,喷施NaHS溶液使盐碱胁迫下裸燕麦千粒质量下降了5.91%,而穗数量、穗铃数量、穗粒数量和籽粒产量分别提高了2.19%、9.70%、61.60%和52.83%。表明外源H_(2)S参与盐碱胁迫下裸燕麦有机酸和激素水平的调控,能够增强裸燕麦适应盐碱胁迫的能力。In order to explore the regulatory effect of exogenous hydrogen sulfide(H_(2)S)on the levels of organic acids and hormones in plants under saline-alkali stress,naked oats(Avena nuda)were used as materials to study the effect of spraying 50μmol·L^(−1) H_(2)S donor sodium hydrosulfide(NaHS)solution on the contents of organic acids and hormones in leaves and yield traits under 3.00 g·kg^(−1) saline-alkali stress.The results showed that saline-alkali stress significantly increased the contents of succinic acid,butenedioic acid,malic acid,glucuronic acid and total organic acids,respectively,but significantly decreased the contents of pyroglutamic acid,jasmonoyl-isoleucine(JA-Ile),trans-zeatin(tZ)and N^(6)-(Δ^(2)-Isopentenyl)adenine(iP)in leaves respectively.Under saline-alkali stress,spraying NaHS solution significantly increased the contents of 3-Hydroxy-3-methylglutaric acid,indole-3-acetic acid(IAA),gibberellin A_(7)(GA_(7)),methyl jasmonate(MJA),iP and IAA/ABA ratio,respectively,but significantly decreased the contents of glucuronic acid,gibberellin A_(3)(GA_(3)),gibberellin A_(4)(GA_(4)),total gibberellins(GAs),1-aminocyclopropanecarboxylic acid(ACC)and ACC/ABA ratio respectively,while it had no significant effect on the contents of succinic acid,butenedioic acid,malic acid,citric acid,malonic acid,pantothenic acid,nicotinic acid,pyroglutamic acid,suberic acid,phenylpyruvic acid,total organic acids,gibberellin A_(1)(GA_(1)),jasmonic acid(JA),JA-Ile,abscisic acid(ABA),tZ,trans-zeatin-riboside(tZR),N6-(Δ2-isopentenyl)adenosine(iPA),total jasmonic acids(JAs),cytokinin(CTK)and the ratios of GAs/ABA,JAs/ABA and CTK/ABA respectively.The principal component analysis showed that spraying NaHS solution significantly increased the contents of organic acid 3-hydroxy-3-methylglutaric acid and hormones MJA,GA_(7),tZ and IAA in leaves of naked oat under saline-alkali stress by 14.31%,41.83%,50.00%,22.97%and 13.02%,respectively;while the contents of organic acids nicotinic acid,glucuronic acid and hormones GA_(4),ACC,t

关 键 词：裸燕麦 硫化氢 盐碱胁迫 有机酸 激素 

分 类 号：Q945.78[生物学—植物学]