肝细胞癌中磷脂酰肌醇蛋白聚糖-3的表达及其临床意义  

Expression and prognostic significance of GPC-3 protein in hepatocellular carcinoma

作　　者：赵桓玉 张瑞瑞[1] 张正卫 吴建[1] 孙苏安[1] ZHAO Huan-yu;ZHANG Rui-rui;ZHANG Zheng-wei;WU Jian;SUN Su-an(Department of Pathology,Huai'an First Hospital Affiliated to Nanjing Medical University,Jiangsu 223399,China)

机构地区：[1]南京医科大学附属淮安第一医院病理科,江苏223399

出　　处：《肝脏》2022年第11期1180-1182,共3页Chinese Hepatology

基　　金：淮安市自然科学研究计划基金(HABZ202107)。

摘　　要：目的探讨磷脂酰肌醇蛋白聚糖-3(glypican-3,GPC-3)蛋白与HCC的相关性。方法选择2014年1月至2015年4月HCC患者91例。取91份肝癌组织和32份癌旁正常肝组织,采用免疫组织化学SP间接法检测GPC-3蛋白及P53蛋白的表达水平,并对其进行相关性分析和预后评价。结果91份肝癌组织中,73份(80.2%)GPC-3细胞染色阳性;32份癌旁肝组织中,2份(6.3%)GPC-3细胞染色阳性,差异有统计学意义(P<0.01)。HCC患者GPC-3阳性组P53阳性率为92.5%,P53阴性率为70.6%(P=0.015)。GPC-3蛋白表达与HCC的组织学分级及TNM分期有相关性(P=0.010、0.011),GPC-3阳性组患者的生存率为9.6%,低于GPC-3阴性组38.9%(P=0.017)。结论GPC-3是诊断HCC的重要标志物,P53与GPC-3表达在肿瘤发生发展过程中可能存在相关通路,GPC-3的表达模式有助于判断HCC的预后。Objective To investigate the expression and clinical significance of glypican-3(GPC-3) protein in hepatocellular carcinoma(HCC) and to investigate the correlation between the GPC-3 expression and the prognosis of HCC. Methods Ninety-one HCC cases and 32 healthy subjects from January 2014 to April 2015 were collected. The GPC-3 and P53 protein levels of all subjects were detected through immunohistochemical SP indirect method. The correlation and prognostic value between HCC and proteins were analyzed. Results The GPC-3 expressed in 80.2% HCC tissues(73/91) and 6.3%(2/32) normal tissues, the difference was. statistically significant(P<0.001). The positive rate P53(92.5%) was significantly higher in GPC-3 positive group(P=0.015). The positive expression of GPC-3 was related to the histological differentiation and TNM stages(P=0.010, P=0.011). The survival rate of patients in GPC-3 positive group(9.6%) was much lower than that in GPC-3 negative group(38.9%)(P=0.017). Conclusion GPC-3 is an important biomarker in diagnosing HCC. P53 and GPC-3 pathway may be related to tumorigenesis and progression of HCC. Detection of the GPC-3 expression is important in evaluating the prognosis of HCC.

关 键 词：肝癌 肝细胞性肝癌 GPC-3 免疫组织化学法 P53 预后 

分 类 号：R735.7[医药卫生—肿瘤]