SPHK1基因对上皮性卵巢癌细胞恶性行为影响及相关机制初步探讨  

The effect of SPHK1gene on malignant behavior of epithelial ovarian cancer cells and its related mechanism

作　　者：赵欢[1] 庞义存[1] 赵郡 邢慧敏[2] 马晓琳 霍翠敏 ZHAO Huan;PANG Yi-cun;ZHAO Jun;XING Hui-min;MA Xiao-lin;HUO Cui-min(Department of Obstetrics and Gynaecology,the Third Hospital of Hebei Medical University,Shijiazhuang 050051,China;The 7th Department of Obstetrics,Maternal and Child Health Hospital,Hebei Province,Shijiazhuang 050006,China;Department of Gynaecology,the Eighth People′s Hospital of Hebei Province,Shijiazhuang 050024,China)

机构地区：[1]河北医科大学第三医院妇产科,河北石家庄050051 [2]河北省石家庄妇幼保健院产七科,河北石家庄050006 [3]河北省第八人民医院妇科,河北石家庄050024

出　　处：《河北医科大学学报》2022年第11期1255-1260,共6页Journal of Hebei Medical University

基　　金：河北省医学科学研究课题计划(20201011)。

摘　　要：目的探讨鞘氨醇激酶1(sphingosine kinase 1,SPHK1)对上皮性卵巢癌细胞生物学行为改变的作用及其相关机制,通过本研究,可为上皮性卵巢癌的早期诊断、预后、耐药治疗、靶向治疗等提供新的依据。方法通过小干扰RNA(siRNA)转染SKOV3细胞干扰SPHK1基因表达后,检测SPHK1表达的高低对于细胞增殖力、细胞克隆形成力以及对于顺铂的敏感性的改变,并检测对于相关干细胞标记物NANOG,耐药相关基因MDR1的表达情况的影响。结果SPHK1基因的表达降低会引起上皮性卵巢癌细胞增殖力的降低、克隆形成力的降低,以及对顺铂的敏感性的增加,影响上皮性卵巢癌细胞中NANOG、MDR1的表达。结论SPHK1可能通过NANOG的表达影响肿瘤干细胞的构成,从而影响上皮性卵巢癌细胞的恶性行为。Objective To explore the effect of sphingosine kinase 1(SPHK1)on the biological behavior changes of ovarian cancer cells and its related mechanisms.Provide new evidence for the early diagnosis,prognosis,drug resistance treatment,and targeted therapy of epithelial ovarian cancer.Methods After transfecting SKOV3 cells with small interfering RNA(siRNA)to interfere with the expression of SPHK1 gene,we detected the effect of the changes of SPHK1 expression on cell proliferation,cell cloning and sensitivity to cisplatin,and also on the expression of stem cell markers NANOG,and the drug resistance-related gene MDR1.Results Decreased expression of SPHK1 gene caused a decrease in the proliferation of ovarian cancer cells,a decrease in clonogenicity,and an increase in sensitivity to cisplatin.The decreased expression of SPHK1 affected the expression of NANOG and MDR1 in ovarian cancer cells.Conclusion SPHK1 may influence the proportion of tumor stem cells through the NANOG,thereby affecting the malignant behavior of epithelial ovarian cancer.

关 键 词：卵巢肿瘤 鞘氨醇 肿瘤干细胞 

分 类 号：R737.31[医药卫生—肿瘤]