基于MXene的黄曲霉素电化学适体传感器的构建  

Construction of aflatoxin electrochemical aptasensor based on MXene

作　　者：陈本奇 于霄 廖文椿 陈志雄 杨梦 李紫滢 孟爽 杨云慧[1] 杨通 胡蓉[1] CHEN Ben-qi;YU Xiao;LIAO Wen-chun;CHEN Zhi-xiong;YANG Meng;LI Zi-ying;MENG Shuang;YANG Yun-hui;YANG Tong;HU Rong(School of Chemistry and Chemical Engineering,Yunnan Normal University,Kunming 650500,China)

机构地区：[1]云南师范大学化学化工学院,云南昆明650500

出　　处：《化学研究与应用》2022年第12期2814-2823,共10页Chemical Research and Application

基　　金：国家自然科学基金项目(21765026)资助。

摘　　要：本文利用黄曲霉素(AFB_(1))与AFB_(1)适体的特异性识别来测定AFB_(1)的含量。采用MXene材料中的TiC纳米材料掺杂金纳米颗粒作为传感基底,以负载金纳米颗粒的金属有机框架材料Au NPs@UiO-66-NH为信号探针,利用杂交作用将标记有Au NPs@UiO-66-NH的AFB_(1)适体链结合到DNA上。采用差分脉冲伏安法(DPV),在Tris-HCl缓冲溶液中测定Au NPs@UiO-66-NH复合材料的DPV响应电流,构建了signal-off型的电化学适体传感器。传感器对AFB_(1)的线性响应范围为0.1~110 ng·mL^(-1),检出限为0.0331 ng·mL^(-1),线性方程为△I=0.5487x+0.9530,实现了对目标物的定量检测。In this paper,the specific recognition of aflatoxin(AFB_(1))and AFB_(1)aptamer was used to determine the concentration of AFB_(1).Au NPs@UiO-66-NH,a metal-organic framework loaded with Au NPs,was used as a signal probe.The AFB_(1)aptamer chain labeled with Au NPs@UiO-66-NHwas bound to DNAthrough hybridization.Differential pulse voltammetry(DPV)was used to measure the DPV response current of Au NPs@UiO-66-NHcomposites in Tris-HCl buffer solution,and a signal-off electrochemical aptasensor was constructed.The linearresponse range of the sensor to AFB_(1)is 0.1~110 ng·mL^(-1),the detection limit is 0.0331 ng·mL^(-1),and the linear equation is△I=0.5487x+0.9530,which realizes the quantitative detection of the target.This work provides a new method for the rapid detection of AFB_(1).

关 键 词：MXene 黄曲霉毒素 Au NPs@UiO-66-NH_(2) 适体传感器 

分 类 号：O657.1[理学—分析化学]