剑叶龙血树内生真菌Colletotrichum sp.B-152次生代谢产物研究  

Study on secondary metabolites of Colletotrichum sp.B-152 derived from Dracaena cochinchinensis

作　　者：张科涛 黄之镨 赵玉瑛 张冰 许小蓉 许玉潇 赵庆 张晓梅 ZHANG Ke-tao;HUANG Zhi-pu;ZHAO Yu-ying;ZHANG Bing;XU Xiao-rong;XU Yu-xiao;ZHAO Qing;ZHANG Xiao-mei(College of Basic Medicine&College of Traditional Chinese Medicine,Yunnan University of Chinese Medicine,Kunming 650500,China)

机构地区：[1]云南中医药大学基础医学院&中药学院,昆明650500

出　　处：《天然产物研究与开发》2022年第12期2056-2062,2097,共8页Natural Product Research and Development

基　　金：国家自然科学基金(21662048);云南省万人计划“青年拔尖人才”专项(YNWR-QNBJ-2019-161)。

摘　　要：采用色谱技术和波谱学分析从剑叶龙血树内生真菌Colletotrichum sp.B-152的培养液和菌丝中,鉴定出了9个化合物:2-hydroxy-4-methyl-1-phenyl-3-pentanone(1)、4-methyl-1-phenyl-2,3-pentanediol(2)、胸腺嘧啶核苷(3)、腺嘌呤核苷(4)、(22 E,24 R)-ergosta-7,22-diene-3β,5α,6β-triol(5)、(4 E,8 E)-N-D-2′-hydroxypalmitoyl-1-O-β-D-glycopyranosyl-9-methyl-4,8-sphingadienine(6)、tuberosider(7)、(22 E,24 R)-5α,8α-epidioxyergosta-6,22-dien-3β-ol(8)、十七烷酸(9),其中化合物1作为天然产物首次报道,化合物2~4、6、7、9首次从刺盘孢属Colletotrichum真菌中分离得到。采用滤纸片扩散法对单体化合物的抑菌活性进行测定,9个化合物对至少一种指示菌表现出不同程度的抑制作用,其中化合物7和8表现出较为广谱的抑菌活性。Nine compounds were isolated and identified from the fermentation of Colletotrichum sp.B-152,which derived from Dracaena cochinchinensis,by chromatographic and spectroscopic analysis.They are 2-hydroxy-4-methyl-1-phenyl-3-pentanone(1),4-methyl-1-phenyl-2,3-pentanediol(2),thymidine(3),adenosine(4),(22 E,24 R)-ergosta-7,22-diene-3β,5α,6β-triol(5),(4 E,8 E)-N-D-2′-hydroxypalmitoyl-1-O-β-D-glycopyranosyl-9-methyl-4,8-sphingadienine(6),tuberosider(7),(22 E)-5α,8α-epidioxyergosta-6,22-dien-3β-ol(8),daturic acid(9).Among which,2-hydroxy-4-methyl-1-phenyl-3-pentanone(1)was reported as natural product for the first time,compounds 2-4,6,7 and 9 were isolated from genus Colletotrichum for the first time.The antimicrobial activities of isolated compounds were determined by paper diffusion method.These 9 compounds showed inhibition abilities against more than one pathogens,compounds 7 and 8 showed broad-spectrum antimicrobial activities.

关 键 词：剑叶龙血树 内生真菌 刺盘孢属 次级代谢产物 抑菌活性 

分 类 号：R284.2[医药卫生—中药学]