番茄红素对秦川牛精液冷冻保存及鲜精品质影响  

Impact of Lycopene on Semen Cryopreservation and Fresh Semen Quality of Qinchuan Bull

作　　者：王萌[1] 杨超群 吴斯林 谭建兵 杜鑫泽 李振兴 昝林森[1] 杨武才[1] WANG Meng;YANG Chaoqun;WU Silin;TAN Jianbing;DU Xinze;LI Zhenxing;ZAN Linsen;YANG Wucai(College of Animal Science and Technology,Northwest A&F University,Yangling 712100,China)

机构地区：[1]西北农林科技大学动物科技学院,杨凌712100

出　　处：《畜牧兽医学报》2022年第12期4507-4517,共11页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：西北农林科技大学试验示范站(基地)科技创新与成果转化项目(TGZX2021-25);陕西省农业科技创新驱动项目(NYKJ-2022-YL(XN)03)。

摘　　要：旨在探究番茄红素对秦川牛精液冷冻保存和新鲜精液品质的影响,为番茄红素作为新型精液稀释液添加剂和种公畜日粮添加剂提供依据。本研究采集3头年龄3~5岁健康状况良好秦川种公牛的新鲜精液混合,用0、1、2、3、4μmol·L^(-1)浓度的番茄红素稀释液冷冻保存;利用计算机辅助分析仪、低渗肿胀检测法、花生凝集素荧光标记检测法、罗丹明荧光检测法等检测精子的运动性能、顶体完整率、质膜完整性、线粒体活性和抗氧化性能;在秦川种公牛日粮中添加15 mg·kg^(-1)BW番茄红素,检测饲喂前后秦川种公牛的新鲜精液品质和血清抗氧化指标。结果表明,在秦川牛精液冷冻保存过程中添加番茄红素可以显著提高冷冻-解冻后精子的活力、顶体完整率、质膜完整性和线粒体活性以及抗氧化酶CAT和GSH-Px酶活性,显著降低精子中MDA含量,且番茄红素的最适添加浓度为2μmol·L^(-1)(P<0.05);饲喂15 mg·kg^(-1)BW番茄红素后,与饲喂之前相比,秦川种公牛的精子活力显著提高(P<0.05),精子畸形率显著降低(P<0.05),血清中抗氧化酶SOD、CAT、GSH-Px酶活性显著增加(P<0.05),MDA含量显著降低(P<0.05)。综上所述,一定浓度的番茄红素对精子在冷冻过程中造成的精子损伤和秦川牛机体应激造成的繁殖性能降低具有良好的保护作用,这可能与番茄红素通过抗氧化作用减少精子在冷冻-解冻过程中的氧化损伤和提高动物机体的抗氧化能力有关。The purpose of this experiment was to investigate the effect of lycopene on the semen cryopreservation and the fresh semen quality,and to provide a basis for lycopene as a new semen diluent additive and dietary additive for breeding males.Fresh semen from 3 healthy Qinchuan bulls aged 3 to 5 years old was collected and cryopreserved with diluents containing lycopene of 0,1,2,3 and 4μmol·L^(-1),respectively.The motility,acrosome integrity,membrane integrity,mitochondrial activity and antioxidant capacity of sperms were measured by computer-aided analyzer,hypo-osmotic swelling test,peanut lectin fluorescent labeling assay,rhodamine fluorescence assay,and so on.Qinchuan bulls were fed with 15 mg·kg^(-1)BW lycopene,fresh semen quality and serum antioxidant indexes were detected before and after feeding.The results showed that the supplementation of the semen extender with lycopene produced a higher sperm motility,acrosome integrity,membrane integrity,mitochondrial activity,CAT and GSH-Px enzyme activities,resulted in a lower content of MDA in sperm than that of the control,the optimal concentration of lycopene was 2μmol·L^(-1)(P<0.05).After 15 mg·kg^(-1)BW lycopene supplementation in diet,compared with before feeding,the sperm motility of Qinchuan bulls had a significant elevation(P<0.05),the sperm deformity rate significantly decreased(P<0.05),and the activities of antioxidant enzymes such as SOD,CAT and GSH-Px significantly elevated(P<0.05),with the content of MDA significantly decreased in serum(P<0.05).In conclusion,a certain concentration of lycopene had a good protective effect on sperm damage caused by semen cryopreservation and the reproductive performance reduction caused by stress in Qinchuan bull,which might be illustrated by the potential function of lycopene on reducing the oxidative damage of sperm during the freezing-thawing process and improving the antioxidant capacity of the animal body.

关 键 词：番茄红素 牛精液冷冻保存 精液品质 血清抗氧化指标 抗氧化作用 

分 类 号：S823.3[农业科学—畜牧学]