Phenolic compounds from Chaenomeles speciosa alleviate inflammation in lipopolysaccharide-treated RAW264.7 macrophages via the NF-κB and MAPK pathways  

作　　者：Fuxia Hu Chao Liu Fengqin Wang Changxin Zhou Maotong Zhu Dongxiao Sun-Waterhouse Zhaosheng Wang 

机构地区：[1]Key Laboratory of Food Processing Technology and Quality Control in Shandong Province,College of Food Science and Engineering,Shandong Agricultural University,Taian 271018,China [2]Key Laboratory of Novel Food Resources Processing,Ministry of Agriculture and Rural Affairs,Key Laboratory of Agro-Products Processing Technology of Shandong Province,Institute of Agro-Food Science and Technology,Jinan 250100,China [3]School of Chemical Sciences,The University of Auckland,Private Bag 92019,New Zealand

出　　处：《Food Science and Human Wellness》2023年第4期1071-1080,共10页食品科学与人类健康（英文）

基　　金：supported by the Key R&D Program of Forestry Shandong Province(2021TZXD014);the Science and Technology Innovation Breakthrough Project of Heze Ctiy(2021KJTP10).

摘　　要：Chaenomeles speciosa(Sweet)Nakai cultivated widely in temperate regions possesses anti-inflammatory properties,however,the underlying molecular mechanisms remain not fully understood.In this study,a purified phenolic extract of C.speciosa rich in chlorogenic acid,procyanidin B1 and catechin(determined by HPLC-Q-TOF-MS/MS)exhibited dose-dependent anti-inflammatory effects on lipopolysaccharide(LPS)-treated RAW264.7 macrophages.The extract at 30μg/mL was most potent and enabled most cells in normal morphology under LPS stimulation without causing cytotoxicity.The extract suppressed the levels of nitric oxide(NO),tumor necrosis factor-α(TNF-α),interleukin-6(IL-6)and interleukin-1β(IL-1β),and the mRNA and protein expressions of nitric oxide synthase(iNOS)and cyclooxygenase-2(COX-2).The mechanisms underlying such anti-inflammatory actions included the regulation of phosphorylation of related proteins to monitor the expressions of inflammatory mediators and genes in the nuclear factor kappa-B(NF-κB)and mitogen-activated protein kinase(MAPK)signaling pathways.Therefore,the phenolic extract from C.speciosa is a desirable anti-inflammatory agent for inflammatory conditions to meet the rising demand for natural and cost-effective therapeutics.

关 键 词：Chaenomeles speciosa Phenolic extract PURIFICATION ANTI-INFLAMMATORY 

分 类 号：TS201.4[轻工技术与工程—食品科学]