Differentially expressed whey proteins of donkey and bovine colostrum revealed with a label-free proteomics approach  

作　　者：Mohan Li Qilong Li Haikun Yu Xiumin Zhang Dehao Li Wanying Song Yan Zheng Xiqing Yue 

机构地区：[1]College of Food Science,Shenyang Agricultural University,Shenyang 110866,China [2]College of Animal Science and Veterinary Medicine,Shenyang Agricultural University,Shenyang 110866,China [3]Beijing Academy of Food Sciences,Beijing 100068,China [4]The Fourth Affiliated Hospital of China Medical University,Shenyang 110866,China

出　　处：《Food Science and Human Wellness》2023年第4期1224-1231,共8页食品科学与人类健康（英文）

基　　金：supported by the by National Key R&D Program of China(2018YFC1604302);“Twelfth Five Year”National Science and Technology Plan Project(2013BAD18B03);Chinese Scholarship Council(202008210391);Shenyang Technological Innovation Project(Y17-0-028);LiaoNing Revitalization Talents Project(XLYC1902083).

摘　　要：This study aimed to analyze and compare the differentially expressed whey proteins(DEWPs)of donkey and bovine colostrum using high-performance liquid chromatography with tandem mass spectrometry-based proteomics.A total of 620 and 696 whey proteins were characterized in the donkey and bovine colostrum,respectively,including 383 common whey proteins.Among these common proteins,80 were identified as DEWPs,including 21 upregulated and 59 downregulated DEWPs in donkey colostrum compared to bovine colostrum.Gene Ontology analysis revealed that these DEWPs were mainly related to cellular components,such as extracellular exosome,plasma membrane,and mitochondrion;biological processes,such as oxidation-reduction process,cell-cell adhesion,and small guanosine triphosphate(GTP)ase-mediated signal transduction;and molecular functions,such as GTP binding,GTPase activity,and soluble N-ethylmaleimide-sensitive factor(NSF)attachment protein receptor activity.Metabolic pathway analysis suggested that the majority of the DEWPs were associated with soluble NSF factor attachment protein receptor interactions in vesicular transport,fatty acid biosynthesis,and estrogen signaling pathways.Our results provide a vital insight into the differences between donkey and bovine colostrum,along with important information on the significant components as nutritional and functional factors to be included in infant formula based on multiple milk sources.

关 键 词：Bovine colostrum Donkey colostrum PROTEOMICS Whey protein Gene Ontology Kyoto Encyclopedia of Genes and Genomes 

分 类 号：TS252.1[轻工技术与工程—农产品加工及贮藏工程]