Investigation on small molecule-aptamer dissociation equilibria based on antisense displacement probe  

作　　者：Lei Wang Lili Yao Qihui Ma Yu Mao Hao Qu Lei Zheng 

机构地区：[1]School of Food and Biological Engineering,Hefei University of Technology,Hefei 230009,China

出　　处：《Food Science and Human Wellness》2023年第4期1257-1264,共8页食品科学与人类健康（英文）

基　　金：supported by the National Key R&D Program of China(2017YFC1600603);the Funds for Huangshan Professorship of Hefei University of Technology(407-037019).

摘　　要：Food safety is a major issue to public health and have attracted global attention.Fast,sensitive,and reliable detection methods for food hazardous substances is highly desirable.Aptamers which can bind to the target molecules with high affinity and specificity represent an attractive tool for the recognition of food hazardous substances,which play an important role in the development and application of new food safety detection technology.But current assays for characterizing small molecule-aptamer binding are limited by either the mass sensitivity or the size differentiation ability.Herein,we proposed a comprehensive method for assessing the dissociation equilibria of small molecule-aptamer,which is immobilized-free under ambient conditions.The design employs the Le Chatelier’s principle and could be used to effectively measure small molecule-aptamer interactions.ATP binding aptamer and anti-aflatoxin B1 aptamer were used as the model system to determine their affinity,in which their dissociation equilibria measurements are in excellent close to their previous work.Due to the simplicity and sensitivity of this new method,we believe that it could be recommended as an effective tool for characterizing small molecule-aptamer interactions and promote the further application of small molecular aptamer in food safety.

关 键 词：APTAMER Small molecule Dissociation equilibria Antisense displacement probe Le Chatelier’s principle 

分 类 号：TS2[轻工技术与工程—食品科学与工程]