Transcriptomics reveals substance biosynthesis and transport on membranes of Listeria monocytogenes affected by antimicrobial lipopeptide brevilaterin B  

作　　者：Yangliu Liu Yawei Ning Zhou Chen Panpan Han Tongxin Zhi Siting Li Aijin Ma Yingmin Jia 

机构地区：[1]School of Food and Health,Beijing Technology and Business University,Beijing 100048,China [2]College of Food Science and Biology,Hebei University of Science and Technology,Shijiazhuang 050018,China

出　　处：《Food Science and Human Wellness》2023年第4期1359-1368,共10页食品科学与人类健康（英文）

基　　金：financially supported by the National Natural Science Foundation of China(31771951,32072199,31801510);the Beijing Natural Science Foundation(KZ201810011016).

摘　　要：Listeria monocytogenes is a worrisome food-borne pathogen threatening global food safety.Our previous study proved that lipopeptide brevilaterin B showed efficient antibacterial activity against L.monocytogenes by interacting with the cell membrane.This research further explored the antibacterial mechanism of brevilaterin B against L.monocytogenes at the sub-minimum inhibition concentration via transcriptomic analysis.Brevilaterin B induced growth inhibition rather than direct membrane lysis in L.monocytogenes at the minimum inhibitory concentration.Transcriptomic analysis showed 1779 difference expressed genes,including 895 up-regulated and 884 down-regulated genes.Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analysis indicated that brevilaterin B influenced multiple pathways of L.monocytogenes,including peptidoglycan biosynthesis,membrane transport(ATP-binding cassette transports,ion transport),cellular metabolism(amino acid and lipid metabolism),ATP synthesis,and activation of the stress response(quorum sensing and bacterial chemotaxis).In conclusion,brevilaterin B affects gene expression related to biosynthesis,transport and stress response pathways on the membrane of L.monocytogenes.The present work provides the first transcriptomic assessment of the antibacterial mechanism of lipopeptide brevilaterin B at the gene level.

关 键 词：Antimicrobial lipopeptide Brevilaterin B Antibacterial mechanism Listeria monocytogenes TRANSCRIPTOMICS 

分 类 号：TS202.3[轻工技术与工程—食品科学]