一株十二烷基硫酸钠高效降解菌的分离鉴定、降解特性及代谢途径研究  

Isolation, Identification, Degradation Characteristics and Metabolic Pathway of an Efficient Sodium Dodecyl Sulfate-degrading Bacterium

作　　者：牛鸿宇 舒倩 杨海君[1] 颜智勇[1] 谭菊 NIU Hong-yu;SHU Qian;YANG Hai-jun;YAN Zhi-yong;TAN Ju(School of Resources and Environment,Hunan Agricultural University,Changsha 410128;School of Plant Protection,Hunan Agricultural University,Changsha 410128;Changsha Ecological Environment Monitoring Center of Hunan Province,Changsha 410001)

机构地区：[1]湖南农业大学资源环境学院,长沙410128 [2]湖南农业大学植物保护学院,长沙410128 [3]湖南省长沙生态环境监测中心,长沙410001

出　　处：《生物技术通报》2022年第12期287-299,共13页Biotechnology Bulletin

基　　金：湖南省自然科学基金项目(2020JJ6059)。

摘　　要：为获得更丰富的十二烷基硫酸钠(sodium dodecyl sulfate,SDS)降解菌资源,从本实验室筛选保存的复合菌系SDS1中,分离出1株以SDS为唯一碳源生长的高效降解菌D2,结合形态学观察、生理生化特征及16S rRNA序列分析,结果显示D2为帕拉伯克霍尔德菌(Paraburkholderia tropica)。实验结果证实,在温度30℃、pH 7、盐度0.1%(W/W)、附加氮源硝酸钠+氯化铵的条件下培养48 h,D2对初始浓度1 200 mg/L的SDS降解率达到98.3%。采用离子色谱法、气相色谱法、液相色谱法对D2在SDS降解过程中的主要代谢产物进行检测与鉴定,初步推测出其对SDS的降解途径可能为SDS→硫酸盐+1-十二烷醇→十二烷醛→十二烷酸→乙酸→CO+HO。本研究可为含SDS表面活性剂废水的高效降解处理提供微生物资源。In order to obtain more abundant resources of sodium dodecyl sulfate(SDS)-degrading bacteria,a highly efficient SDSdegrading strain D2 with SDS as the only carbon source was isolated from the composite strain SDS1 screened and preserved in our laboratory.According to its morphological characteristics,physiological and biochemical experiments and 16S rRNA gene sequence molecular analysis,D2 was identified as Paraburkholderia tropica. The experimental results confirmed that the degradation rate of SDS by D2 increased to 98.3% at a temperature of 30℃,pH 7,salinity 0.1%(W/W)when the initial concentration of SDS was 1 200 mg/L,and an additional nitrogen source of sodium nitrate + ammonium chloride for 48 h. The main metabolites of the strain in SDS degradation were detected via ion chromatography,gas chromatography and liquid chromatography. The feasible degradation pathway of SDS by D2 might be SDS → sulfate +1-dodecanol →dodecanaldehyde → dodecanoic acid → acetic acid → CO+ HO. This study may provide microbial resources for the efficient degradation of SDS-containing surfactant wastewater.

关 键 词：十二烷基硫酸钠 帕拉伯克霍尔德菌 微生物降解 分离鉴定 降解特性 代谢途径 

分 类 号：X172[环境科学与工程—环境科学] X703