不同生长模式早产儿血清代谢组学分析  

Analysis of serum metabolomics of premature infants in different growth patterns

作　　者：陈莹 王玉美[2] 徐文英[1] 张佩滢 CHEN Ying;WANG Yumei;XU Wenying;ZHANG Peiying(Department of Child Health Care,Huai’an Maternal and Child Health Hospital Affiliated to Yangzhou University Medical College,Jiangsu Province,Huai’an223002,China;Department of Neonatal Disease Screening,Huai’an Maternal and Child Health Hospital Affiliated to Yangzhou University Medical College,Jiangsu Province,Huai’an223002,China)

机构地区：[1]扬州大学医学院附属淮安市妇幼保健院儿童保健科,江苏淮安223002 [2]扬州大学医学院附属淮安市妇幼保健院新生儿疾病筛查科,江苏淮安223002

出　　处：《中国医药导报》2022年第33期24-28,共5页China Medical Herald

基　　金：江苏省青年医学重点人才培养项目(QNRC2016440);江苏省淮安市自然科学研究计划项目(HAB201937)。

摘　　要：目的 应用代谢组学方法分析不同生长模式早产儿血清代谢物特征,并寻找差异代谢物及代谢通路。方法 采集2019年3月至2020年12月扬州大学医学院附属淮安市妇幼保健院新生儿重症监护病房住院且儿童保健科门诊随访至校正月龄6月龄的55例早产儿血清标本,根据校正月龄6月龄与出生体重Z评分差值是否≥0.67分为追赶生长组(35例)和无追赶生长组(20例)。应用液相色谱-串联质谱联用技术检测血清代谢谱,比较两组血清代谢轮廓的差异,筛选、鉴定出组间具有显著差异的代谢物及代谢通路。结果 共得到28种主要差异代谢物,其中上调物质27种,主要为氨基酸类、甘油磷脂类、有机杂环化合物等;下调物质1种,属脂肪酸类。代谢物差异通路主要集中在氨基酸的代谢与合成中,其中富集程度最显著的代谢通路为精氨酸和脯氨酸代谢及谷胱甘肽代谢。结论 早期发生追赶生长的早产儿,因其生长速度较快,更易出现各种营养物质尤其是氨基酸的缺乏,应重视早产儿氨基酸的合理补充。代谢组学分析可以为早产儿的营养状况评估和干预提供科学依据。Objective To analyze the characteristics of serum metabolites and to search for differential metabolites and metabolic pathways of preterm infants in different growth patterns using metabolomics techniques. Methods Serum samples were collected from 55 preterm infants by six months of adjusted age who were hospitalized in Neonatal Intensive Care Unit and were followed by Department of Child Health Care of Huai’an Maternal and Child Health Hospital Affiliated to Yangzhou University from March 2019 to December 2020. They were divided into catch-up growth group(35 cases) and without catch-up growth group(20 cases) according to whether the Z-score difference between corrected age of six months and birth weight was ≥0.67. The serum metabolic profiles were detected by liquid chromatography-tandem mass spectrometry, the metabolites and metabolic pathways with significant differences between two groups were identified,respectively. Results A total of 28 serum differential metabolites were obtained. Among them, there were 27 upregulated substances, mainly amino acids, glycerol phospholipids, and organic heterocyclic compounds, etc.;down-regulated substance 1, belonging to fatty acids. The metabolite difference pathways mainly concentrated in metabolism and synthesis of amino acids. The most significant enrichment pathways were arginine and proline metabolism and glutathione metabolism.Conclusion Premature infants with catch-up growth in the early stage are more prone to lack of various nutrients, especially amino acids, because of their rapid growth. It should pay attention to the reasonable supplement of amino acids for preterm infants. Metabonomics analysis can provide scientific basis for nutritional evaluation and intervention of preterm infants.

关 键 词：代谢组学 追赶生长 早产儿 氨基酸 癸酰肉碱 

分 类 号：R725.8[医药卫生—儿科]