食管癌患者血清miR-194水平及癌组织中KDM5B表达水平与食管癌临床病理特征的关系  

Expression levels of KDM5B and miR-194 in esophageal cancer and the relationship with clinicopathological characteristics of patients

作　　者：洪永贵[1] 胡彦伟 徐露娟 武伟龙 杨海军[1] Hong Yonggui;Hu Yanwei;Xu Lujuan;Wu Weilong;Yang Haijun(First Department of Internal Medicine,Anyang Cancer Hospital,Anyang Henan 455000,China)

机构地区：[1]安阳市肿瘤医院内一科,河南安阳455000

出　　处：《保健医学研究与实践》2022年第11期41-46,51,共7页Health Medicine Research and Practice

基　　金：河南省医学科技攻关计划项目(LHGJ20191280)。

摘　　要：目的探讨组蛋白去甲基化转移酶(KDM5B)、miR-194在食管癌患者中表达水平及与食管癌临床病理特征的关系,以期为临床诊治提供参考。方法选取2020年1—6月在安阳市肿瘤医院接受手术治疗的96例食管鳞癌患者作为观察组,通过手术取得食管癌患者的癌组织和距离癌组织3 cm以上的癌旁组织。另外选取40例健康人作为对照组。采用免疫组化法检测KDM5B在食管癌组织、癌旁组织中的表达情况;采用实时荧光定量聚合酶链反应(PCR)检测观察组和对照组研究对象血清miR-194水平;采用多因素Cox回归分析影响食管癌患者预后的危险因素。结果食管癌组织的KDM5B阳性表达率明显高于癌旁组织,差异有统计学意义(χ~2=9.632,P=0.007);对照组研究对象血清miR-194水平明显低于观察组,差异有统计学意义(Z=14.306,P<0.001)。临床分期为Ⅲ期、浸润深度为T3~T4、有淋巴结转移的食管癌患者KDM5B阳性表达率、miR-194表达水平高于临床分期为Ⅰ~Ⅱ期、浸润深度为Tis~T2、无淋巴结转移的食管癌患者,差异有统计学意义(P<0.05)。随访2年后,共有40例患者存活,存活率为41.67%(40/96)。KDM5B阳性表达患者的2年生存率(32.31%,21/65)低于KDM5B阴性表达患者(61.29%,19/31),差异有统计学意义(P<0.05)。miR-194高表达组患者的2年生存率(31.25%,15/48)低于miR-194低表达组患者(52.08%,25/48),差异有统计学意义(P<0.05)。临床分期为Ⅰ~Ⅱ期、浸润深度为Tis~T2、无淋巴结转移、KDM5B阴性表达、miR-194低表达的食管癌患者2年生存率高于临床分期为Ⅲ期、浸润深度为T3~T4、有淋巴结转移、KDM5B阳性表达、miR-194高表达的食管癌患者,差异均有统计学意义(P<0.05)。浸润深度为T3~T4、有淋巴结转移、KDM5B阳性表达、miR-194高表达是食管癌患者2年生存率的危险因素(P<0.05)。结论KDM5B、miR-194表达水平与食管癌患者的临床分期、浸润深度、是否有淋巴结转移密�Objective To investigate the expression of lysine-specific histone demethylase 5B(KDM5B)and miR-194 in esophageal carcinoma and the relationship with clinicopathological characteristics of patients to provide a reference for clinical diagnosis and treatment.Methods Ninety-six patients with esophageal squamous cell carcinoma who underwent surgery in Anyang cancer hospital from January to June 2020 were selected as participants in the observation group.Another 40 healthy individuals were selected as controls.Immunohistochemistry was used to detect the expression of KDM5B in esophageal cancer tissues and paracancerous tissues.Real-time polymerase chain reaction(PCR)was employed to detect the serum miR-194 level in the observation group and healthy participants group.Multivariate Cox regression was adopted to analyze the risk factors affecting the prognosis of patients with esophageal cancer.Results The positive expression rate of KDM5B in esophageal carcinoma tissues was significantly higher than that in paracancerous tissues(χ~2=9.632,P=0.007),and the serum miR-194 level in the control group was significantly lower than that in the observation group(Z=14.306,P<0.001).The positive expression rate of KDM5B and the expression level of miR-194 in patients with clinical stageⅢesophageal cancer and a depth of invasion T3~T4 and lymph node metastasis were significantly higher than those in patients with clinical stageⅠ~Ⅱesophageal cancer,depth of invasion Tis~T2,and no lymph node metastasis(P<0.05).After 2 years of follow-up,a total of 40 patients survived,with a survival rate of 41.67%(40/96).The 2-year survival rate of patients with positive KDM5B expression(32.31%,21/65)was significantly lower than that of patients with negative KDM5B expression(61.29%,19/31,P<0.05).The 2-year survival rate of patients in the high miR-194 expression group(31.25%,15/48)was significantly lower than that of patients in the low miR-194 expression group(52.08%,25/48,P<0.05).The 2-year survival rate of patients with clinical stageⅠ~

关 键 词：组蛋白去甲基化转移酶 miR-194 食管癌 2年生存率 

分 类 号：R735[医药卫生—肿瘤]